成果報告書詳細
管理番号20110000000684
タイトル*平成22年度中間年報 微生物機能を活用した環境調和型製造基盤技術開発/微生物群のデザイン化による高効率型環境バイオ処理技術開発/有用微生物群による高効率好気水処理技術の研究開発」
公開日2011/6/7
報告書年度2010 - 2010
委託先名株式会社日立プラントテクノロジー
プロジェクト番号P07024
部署名バイオテクノロジー・医療技術部
和文要約和文要約等以下本編抜粋:1.研究開発の内容及び成果等 活性汚泥法では多様な自然発生した微生物群により好気的に有機物を分解する。好気反応では膨大な曝気動力が必要であり多大なランニングコストを必要とする。そこで本研究では,酸素を消費する微生物フローラ側に視点をおき,酸素をあまり必要としない理想的好気フローラを人為的に構築することにより必要酸素量の低減化を試みている。 平成22年度~23年度では,4研究機関の連携を図りメタン発酵部と亜硝酸型硝化,更にアナモックスからなる窒素処理部を組み合わせた一体処理型廃水処理システムによるアンモニア態窒素を含む有機系廃水処理の実証評価を行う。その中で日立プラントテクノロジーは,亜硝酸型硝化に関わる研究開発を担当し,目的外の微生物を担体から排除するアルカリ処理技術を活用した亜硝酸型硝化処理性能を検証する。
英文要約Annual report 2011: High efficiency wastewater treatment using novel nitritation system with alkaline-shocked gel carriers.
Hitachi Plant Technologies, Ltd. & Chuo University
The energy and cost expenses for aeration in an activated sludge plant are intensive and tremendous, of which fraction often exceeds a half of total cost expenses of the plant. As it has been long desired to reduce this heavy burden of expenses for aeration, we are pursuing to develop a novel nitritation process based on the observation that nitrite-oxidizing bacteria (NOB) in sewage sludge can be killed by strong alkaline-shock, but ammonium-oxidizing bacteria (AOB) would survive. To evaluate the nitritation performance, continuous feeding tests were conducted using alkaline-shocked gel carriers.
(1) Selection of a wastewater treatment plant which could supply wastewater with high ammonia for our examination: Dewatering liquid of digested sludge was selected for evaluating the nitritation performance using alkaline-shocked gel carriers. We chose a sludge treatment center in Yokohama, where produces wastewater with the highest concentration of ammonia among plants we have surveyed.(2) Nitritation treatment of liquid digested sludge.: It has been confirmed so far that operation start-up for nitritation activity is completed within ten days and nitrification restraining nitratation activity is sustained for two month. Production rate of nitritation is increased up to 0.79 kg-N/(m3・d) at a maximum. (3) Effect of free-ammonia concentration on nitratation.: It has been confirmed that free-ammonia restrains nitratation activity. However this effect has been found to vary according to the concentrations of nitrite oxidizing bacteria.(4) Development of GC/MS method for the determination of gaseous metabolites: We developed a simple and rapid method for the simultaneous quantification of a variety of gas species. In this method, the injection port and the sampling port were entirely covered with He stream to prevent the contamination of ambient gases. Concentration of N2O in the sample was precisely and sensitively determined with this method.(5) Effect of the alkaline-shock treatment to N2O transformation ratio from NH4, its substrate: N2O gas sampled from the headspace of the plant before and after alkaline-shock treatment and analyzed by the developed method described above. After alkaline-shock treatment, N2O/NH4 transformation ratio, which is the ratio of N2O-N concentration in the removed NH4-N concentration, was decreased from that determined before alkaline-shock treatment. N2O/NH4 transformation ratio did not correlate with aeration ratio as far as it changed between 0.4 and 4.0 L/min/L-tank.(6) Preliminary studies on analyses on AOB and Archaeal community structure: DNA was extracted from gel carriers carrying nitrifying community taken before and after alkaline-shock treatment. 16S rRNA genes of AOB and Archaea were PCR amplified by β-proteobacterial AOB-specific and Archaea-specific primers, respectively, cloned, and sequenced. Nitrosomonas europaea predominated in AOB community of before and after alkaline-shock treatment. Archaeal community composition analysis is forthcoming.
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