成果報告書詳細
管理番号20110000000687
タイトル*平成22年度中間年報 微生物機能を活用した環境調和型製造基盤技術開発/微生物群のデザイン化による高効率型環境バイオ処理技術開発/バイオフィルム工学による微生物のデザイン化
公開日2011/6/7
報告書年度2010 - 2010
委託先名国立大学法人北海道大学地球環境科学研究院
プロジェクト番号P07024
部署名バイオテクノロジー・医療技術部
和文要約和文要約等以下本編抜粋:1. 研究開発の内容及び成果等 (1) 硝化槽における微生物群のデザイン化:従属栄養細菌群との相互作用を利用した好気的アンモニア酸化細菌の活性促進技術の開発 -硝化槽微生物の未知相互作用の解析- 本研究項目では、活性汚泥法の効率化・安定化を目指す。具体的には硝化槽の性能の鍵となる好気的アンモニア酸化細菌について、活性汚泥中で共存する従属栄養細菌群による影響を明らかにし、これらを最適化することによって硝化槽微生物群をデザイン化し、硝化槽の効率化および安定化を目指す。本年度では、代表的なアンモニア酸化細菌Nitrosomonas europaea IFO14298 (以下「IFO株」とする) のアンモニア酸化活性を向上させるMR3が生産する活性化因子について検討した。
英文要約Title: Trial to design microorganisms by engineering biofilms (FY2007-FY2011) FY2010 Annual Report
This project aims to understand and develop the biofilm formation of useful bacteria for effective treatments of industrial wastes. Following results were obtained in this year. (1) Wastewater treatment of nitrogen compounds-1: We had already isolated bacterium Reinheimera sp. MR3 that enhances ammonia-oxidizing activity of Nitrosomonas europaea IFO. It was suggested that MR3 produced IFO activating factors that are permeable to dialysis bag (MWCO>10,000). (2) Wastewater treatment of nitrogen compounds-2: In order to identify cell surface proteins that are responsible for granule formation by Anammox bacterium, Brocadia anammoxidans, cells were treated with proteinase K and five product peptides were obtained by HPLC. (3) Treatment of oil contaminants in soils: We have isolated a new alkane degrading and biofilm forming Acinetobacter sp. LB’-3. It was demonstrated that it degraded alkanes in a crude oil contaminated soils faster when it formed biofilms than planktonic cells. It was also found that community structure of soil bacteria was recovered after 3 weeks of LB’-3 inoculation to the soils. These results support an idea that biofilms are useful for effective and safe treatment of soil contamination. (4) Wastewater treatment of organic compounds: We have successfully developed stable phenol and alkane degrading systems by optimizing the combination of duckweed and its biofilm forming rhizobacteria. We also found that these rhizobacteria were capable of enhancing the growth of duckweed.
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