成果報告書詳細
管理番号20110000000997
タイトル*平成21年度中間年報 基礎研究から臨床研究への橋渡し促進技術開発/橋渡し促進技術開発/細胞シートによる多施設臨床研究を目指した基盤システムの構築
公開日2011/8/30
報告書年度2009 - 2009
委託先名国立大学法人大阪大学医学部付属病院 国立大学法人東北大学 学校法人東京女子医科大学 川崎重工業株式会社 株式会社セルシード
プロジェクト番号P07022
部署名バイオテクノロジー・医療技術開発部
和文要約和文要約等以下本編抜粋:1. 研究開発の内容及び成果等 (1)細胞シートによる心筋再生治療法の普及に向けた研究開発 (実施体制:大阪大学~共同実施 テルモ株式会社、大阪大学~共同実施 千葉大学、東京女子医科大学、株式会社セルシード) (1)-1 心筋再生細胞シートの規格化 筋芽細胞純度と成長因子分泌量を指標とし、より成長因子分泌能の高い細胞純度条件や、温度感受性培養皿への播種密度、播種条件、細胞シートの剥離条件等を検討した。さらに、輸送・保存に伴うこれらの条件変化について検討を行った。
英文要約Title; Translational Research Promotion Project for Multi-center Clinical Research of Regenerative Medicine using Cell Sheet Engineering
Author; Osaka University, Tohoku University, Tokyo Women’s Medical University, Kawasaki Heavy Industries, Ltd., CellSeed Inc.
We have developed a novel strategy for regenerative medicine to recover tissue functions by using temperature-responsive cell culture surfaces. We have also begun clinical research of myoblast sheet transplantation for sever heart failure and corneal epithelial cell sheet for ocular surface disease. In order to standardize and spread the regenerative medicine using cell sheet engineering, it would be required to develop the validation, preservation and transportation method for these cell sheets. In this project, we aimed to develop basic technology for multi-center clinical research of regenerative medicine using cell sheet engineering.
First, we have decided the standard of myoblast sheet about size and cell density. Moreover, we have succeeded in isolated myoblast from muscle tissue stored at 4 degrees C for 24 hour, and decided the condition of preservation.
Second, we have developed a validation system for cultivated epithelial cell sheets using Frequency Domain Optical Coherence Tomography (FD-OCT) and transepithelial electric resistance (TER) measurement. We have also developed the evaluation method of cell sheet using a transgenic rat with firefly luciferase to investigate the condition of preservation and transportation.
Third, the gene mutation of myoblast sheet during in vitro culture, preservation and transportation were investigated. Moreover, the automated culture system (R-CPX), manufactured by Kawasaki Heavy Industries, Ltd., was improved and adapted the culture protocols for the myoblast and corneal epithelial cell.
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