成果報告書詳細
管理番号20110000001154
タイトル*平成22年度中間年報 ナノテク・先端部材実用化研究開発/高機能性蛍光磁性ビーズによる高速・高感度疾患診断システムの開発(2)
公開日2011/9/9
報告書年度2010 - 2010
委託先名多摩川精機株式会社
プロジェクト番号P05023
部署名電子・材料・ナノテクノロジー部
和文要約和文要約等以下本編抜粋:1. 研究開発の内容及び成果等 東京工業大学半田研究室で開発された高機能性蛍光磁性ビーズ(FFビーズ)は疾患マーカの検出を行うために最も重要な材料である。このFFビーズを大量合成し安定供給することが本開発の一つの目標である。昨年度は、多摩川精機において遠心濃縮機とマルチラベルリーダを導入して半田研究室と同様にFFビーズの製造及び評価ができる体制を整えた。また、半田研究室からFFビーズの製造方法を習得して、小スケールで再現性よく製造できるようになった。本年度はFFビーズの量産化のために大量製造法の確立を目標に、スケールアップによるFFビーズの合成について検討を行った。
英文要約Development of mass production method of highly functionalized fluorescent ferrite beads (Tamagawa Seiki Co., Ltd.): Highly functionalized fluorescent ferrite beads (FF beads) developed at Dr. Handa's laboratory, Tokyo Institute of Technology are the most important materials for disease-related marker detection. We at Tamagawa Seiki aim mass production and stable supply of the FF beads. As the first step in this year, for mass production of the FF beads, we evaluate fluorescent substance (europium complex) which is encapsulated in the FF beads. As the results of comparison between commercial fluorescent substance and our self-made one, absorbance and fluorescent intensity of their two substances were closely equal. Furthermore, fluorescent intensity of the FF beads encapsulating the commercial fluorescent substance is also equal to that of the FF beads encapsulating the self-made substance. Therefore, we decided to use the commercial fluorescent substance hereafter, because the self-made fluorescent substance had problems such as unclear purity, low recovery rate and high time cost. As the next step, we investigated mass production of the FF beads. We finally aim several-gram-scale production of the FF beads for several million tests of marker detection. As the result of 100-mg-scale production scaled up from 5-mg-scale production using a centrifugal concentrator in a concentration process, precursor FG beads were dried and precipitated during the process. The fluorescent intensity of prepared FF beads was equal to half of that of the FF beads prepared by 5-mg-scale production. It was considered that the produced FF beads were mixed with the beads encapsulating little fluorescent substance due to drying and precipitation of the beads. In order to resolve the problem, we examined use of a rotary evaporator instead of the centrifugal concentrator in the concentration process. A rotary evaporator can process ca. 8 g of FF beads whereas a centrifugal concentrator up to 1 g. As the result of 100-mg-scale production using the rotary evaporator, precursor FG beads were not dried and precipitated during the concentration process, and we reproducibly prepared the FF beads with the fluorescent intensity which was equal to that prepared by 5-mg-scale production. Next, we tried to prepare the FF beads by 1-g-scale production. As the result, the FF beads were also produced without drying and precipitation of the precursor FG beads and had the fluorescent intensity which was equal to that prepared by 100-mg-scale production. For the future, we will develop several-gram-scale mass production method of FF beads with high magnetic responsibility. We will also investigate optimal immobilization method of antibody on FF beads for disease-related marker detection.
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