成果報告書詳細
管理番号20120000000638
タイトル*平成23年度中間年報 ヒト幹細胞産業応用促進基盤技術開発/ヒト幹細胞実用化に向けた評価基盤技術の開発(浜松ホトニクス株式会社)
公開日2012/9/11
報告書年度2011 - 2011
委託先名浜松ホトニクス株式会社
プロジェクト番号P10027
部署名バイオテクノロジー・医療技術部
和文要約和文要約等以下本編抜粋:1
. 研究開発の内容及び成果等
ヒトES細胞の状態を培養下で生きたまま検定するための、多パラメータ観察技術などのイメージング技術による生細胞検査技術の開発(京都大学、浜松ホトニクス、リプロセル)
増殖させたヒトES 細胞の状態と特性を評価検定し、理想的な状態の標準的なヒトES細胞を供給するため、経時的に、連続して、培養増殖中のヒトES細胞の状態をモニターし、細胞の状態変化や自発的分化細胞の出現などの培養中の不具合を素早く検出することを可能とするイメージング技術による生細胞検査技術の開発を行う。
英文要約Title: Beyond the Fusion of Academia and Industry in Japan: an Integrated System for High Quality and Large Scale Production of hPSCs(FY2011-FY2016)FY2011-2012 Annual Report
1. Development of robust, mass culture and cryo-preservation technologies for hPSC lines
2. Development of quality evaluation and control for hPSC lines
The aim of this NEDO (New Energy and Industrial Technology Development Organization) Project is the “Development of Core Technologies for Industrial Applications of Human Stem Cells”. In recent years, great progress has been made worldwide in developing drug discovery screening and cell therapy applications for hPSCs (human pluripotent stem cells, such as hES cells).However, large scale PSC culture methods vary widely across the globe, and are in need of standardization. Initiated last year in Japan, this six-year national project supported by METI (the Ministry of Economy, Trade and Industry) aims to develop not only large scale production methods, but also the technologies necessary for stem cell evaluation.
1. Development of robust, mass culture and cryo-preservation technologies for hPSC lines
(4) Multi-parameter imaging technology for living cell in the culturing.
To manufacture and provide “good” hESCs, continuous monitoring system for living cultured hESCs are requested. This year, the prototype of imaging system which can obtain multi-parameters without label has been designed and assembled. This imaging system can measure solid profiles of ES cell colony such like optical thickness and adhesive characteristics, two dimensional profile such like perimeter or area of ES cell colony by transparent light imaging. Additionally, the imaging system can measure distribution of molecules which are fluorescent labeled, and also can find all of these temporal and special change.
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