成果報告書詳細
管理番号20120000000494
タイトル*平成23年度中間年報 新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発(先導技術開発) 遺伝子組換えによるバイオマスエネルギー高生産樹木の創生に関する研究開発
公開日2012/12/26
報告書年度2011-2011
委託先名日本製紙株式会社 国立大学法人東京農工大学 国立大学法人筑波大学
プロジェクト番号P07015
部署名新エネルギー部
和文要約和文要約等以下本編抜粋:1. 研究開発の内容及び成果等
本プロジェクトにおいては、遺伝子組換えの手法を用いて不良環境においても生産性の高い樹木を創生する。環境ストレス耐性等を付与することにより、不良環境においてもバイオマスの生産力が維持できる樹木の選抜、評価を目指す。耕作不適の原因として乾燥が挙げられ、塩類の集積による塩害が問題となっている。
英文要約Title: New Energy Technology Development. Development of Technology for High-Efficiency
Conversion of Biomass and Other Energy. R&D of Genetic Engineering to Produce Trees with High
Biomass Energy Productivity
The environmental destruction and a depletion of fossil fuels have become major social problems on
a global scale. Forestation is considered to be an effective approach to mitigate these constraints
because it can preserve the environment and supply raw materials at the same time. In response, it is
important to breed environmental stress-tolerant and fast-growing trees. Our purpose is to produce
trees with high biomass energy productivity that can grow on marginal lands.
1. Development of Harsh-environment-compatible Technology
To increase biomass production under stressed environment conditions, transgenic eucalypts that
induce salt-tolerant gene(s) (mangrin, coline oxidase [codA] and RNA binding protein [RBP] ) have
been produced and selected on the salt medium.These lines were evaluated by salt-tolerance in a
growth room. The salt-tolerant lines were selected from three types of transgenic lines.
2. Studies on Developing Highly Functional Trees
We compared two terminators (HSP and NOS) using the GUS gene. Higher GUS expressions were
observed in the eucalyptus shoot with HSP terminator. To produce highly stress-tolerant trees, the
vector containing codA genes with HSP terminator introduced into eucalyptus. The glysinebenaine
accumulation was determined and higher content were observed in the HSP terminator lines.
3. Research on Mode of Action and Comparative Study of Salt-tolerance Genes
Juncus sp. is a highly salt-tolerant halophyte. To isolate genes on the salt tolerance, functional
screening was performed using E. coli MG1655 as a host organism. In this screening, a cDNA
encoding proline rich protein (JuPRP) was obtained. Transgenic A. thariana callus expressing
JuPRP was constructed. Enhanced salt tolerance was observed in the transgenic callus
expressing JuPRP. JuPRP may play a key role for the salt tolerance in the Juncus sp.
4. Development of Evaluation Methods on GM Trees in Special Netted-house and Field Testing.
Three categories of eucalyptus transgenic genotypes, either with codA, RBP or mangrin gene, were
used as the candidates for establishing salt-tolerant transgenic eucalyptus tree varieties. In FY2011,
we redesigned salt treatment condition for the assay for salt-tolerant trait of transgenic eucalyptus
plants in the special netted-house, and performed pilot experiments. On the results, two transgenic
eucalyptus lines, a codA transgenic line and a mangrin transgenic line, showed significant
perseverant salts-tolerant than non-transgenic eucalyptus on this salt-treatment condition. In addition,
we assessed the impact of RBP transgenic eucalyptus lines for soil microorganism communities and
allelopathic activities of leaves, and showed no significant differences among non-transgenic and
RBP transgenic lines.
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