成果報告書詳細
管理番号20120000000641
タイトル*平成22年度及び23年度中間年報 ヒト幹細胞産業応用促進基盤技術開発/ヒト幹細胞実用化に向けた評価基盤技術の開発(日産化学工業株式会社)
公開日2012/12/20
報告書年度2010-2011
委託先名日産化学工業株式会社
プロジェクト番号P10027
部署名バイオテクノロジー・医療技術部
和文要約和文要約等以下本編抜粋:[記載項目]
1. 研究開発の内容及び成果等
研究開発項目
(1)ヒトES細胞の安定な培養・保存技術の開発
研究者の手技の違いを排除し、一定条件での安定した培養操作を可能とする自動培養技術ヒトES細胞の有用な性質を損なわずに安定培養が可能な、成分が明確かつ異種生物由来の成分を含まない培養液・培養基板の開発、凍結融解後の回収率が高く、ユーザーへの提供方法を考慮した凍結保存技術の開発を目的に、以下の技術開発を行なう。
英文要約Title: Development of Core Technologies for Industrial Applications of Human Stem Cells
Consigned company:Nissan Chemical Industries, LTD.
Introduction :The aim of this NEDO (New Energy and Industrial Technology Development Organization) Project is to develop the core technologies for industrial applications of human stem cells. In recent years, great progress has been made worldwide in developing drug discovery screening and cell therapy applications for hPSCs (human pluripotent stem cells, such as hES cells).
However, large scale PSC culture methods vary widely across the globe, and are in need of standardization. Initiated national project supported by METI (the Ministry of Economy, Trade and Industry) aims to develop not only large scale production methods, but also the technologies necessary for stem cell evaluation.
Research Plan and Targets (2011)
Development of robust, mass culture and cryo-preservation technologies for hPSC lines
(1) Synthetic, defined culture medium with low molecular weight chemical compounds
(2) Culture substrate and materials for large scale cell culture systems
(3) Closed and minimal risk cell culture system and 3D mass culture technologies
Progress situation (2011)
Materials under development: Chemicals, Substrates and Reagents
Progress in robust, mass culture for hPSCs depends on the development of synthetic, defined medium with low molecular weight chemical compounds. We first developed screening system to identify novel small molecules for the effect in culture of hPSCs. Then, we started screening with various chemical libraries. Our initial results identified novel chemicals which have potentially allows the expansion of hPSCs efficiently.
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