成果報告書詳細
管理番号20120000000923
タイトル*平成23年度中間年報 新エネルギー技術研究開発/バイオマスエネルギー等高効率転換技術開発(先導技術開発)/酵素糖化・効率的発酵に資する基盤研究
公開日2012/12/26
報告書年度2011-2011
委託先名一般財団法人バイオインダストリー協会
プロジェクト番号P07015
部署名新エネルギー部
和文要約和文要約等以下本編抜粋:1.研究開発の内容および成果等
加速的先導技術開発は,2015-2020 年頃バイオマスエネルギーの普及に重要とされるセルロース系バイオマス資源からバイオエタノールを40 円/リットルで製造することや軽油代替燃料開発ための一貫プロセス開発を目的としている。
英文要約Title: Basic Research and Development on Enzymatic Saccharification of Cellulosic Resources and Biofuel Production : (FY 2008-FY 2012)
FY2011 Annual Report
This R & D objective is to develop innovative means for converting renewable lignocellulosic biomass to practical liquid fuel, bioethanol. To achieve this, the R&D has been implemented for selecting enzymes, proteins and genes for improving saccharification of biomass and breeding of C6/C5 fermenting yeast for bioethanol to support the integrated process development group.
The fourth fiscal year (2011/4-2012/3), 16 contractors conducted jointly the following three R & D themes.
R & D Theme v) "Achievement of highly effective saccharification by breakthrough of saccharification system"
Understandings of composition and structure of biomass, crystallinity of cellulose, their structural change by pretreatment, interactions among the component enzymes of the saccharification enzyme and pretreated biomass, a decrease of the enzyme amounts used, a high density saccharification, and the enzyme recovery and recycling are proceeded targeting entire breakthrough of saccharification system.
In 2011, the emphasis was put on the development of the technique for achieving a concrete, highly effective saccharification based on the results until last year. As a result, "The hitting of the ceiling", the phenomenon of reaching the saccharification limit when used low enzyme concentrations, that was of the biggest concern for saccharification, was able to be eased or canceled by adding surplus surfactants or proteins. From this result, the search for additives with economically feasible and the improvement of the enzyme by clarifying the function of the additives are now being carried out. The highly density saccharification methods and the courses of the enzyme improvement could be clarified from the analysis of the adsorption phenomenon of the enzyme to the biomass when saccharifying it. In addition, it was clarified that the saccharification obstruction by glucose accumulated was due to an inhibition of cellulase but not BGL with glucose when used the saccharification enzyme with high activity of BGL.
R & D Theme vi) "Creation of innovative saccharification enzyme"
The component enzymes and proteins from other organisms that show better performance in saccharification than those of the component enzymes from Trichoderma reesei are screened out, collected and evaluated. The innovative Trichoderma saccharification enzyme that contains the best component enzymes in the best ratio is made and a cheap mass production method of the enzyme is developed in large scale, and it will be provide to domestic bioethanol-developing teams.
The following results were achieved in fiscal year 2011.
The search for the component enzymes and proteins from wide-ranging and various origins had been well advanced. Several CBH’s and a number of hemicellulases were obtained which are equal or better than those of Trichoderma reesei. In addition, new evaluation systems became possible from the result of R & D theme v), and constructing a lot of component deficient enzymes was succeeded for the decision of the ratio of the component enzymes, and they are being also used for the above-mentioned component enzyme evaluation.
From the evaluation test of the high performance saccharification enzyme JN13 (and JN11) that were created till last year, they surpassed completely Cellic CTec2 (Novozymes) with the best performance in the world now. Moreover, it turned out that JN11 and JN13H (H is a hemicellulase-reinforced enzyme) should be properly selected according to kinds of pretreatment biomasses. The mass production method in the jar culture and the tank fermentation is examined, and 1.75 kg-protein(enzyme) was acquired by culture with 200 l fermentation tank. It will be offered to other teams and other organizations for evaluation.
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