成果報告書詳細
管理番号20120000000937
タイトル*平成23年度中間年報 新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発(先導技術開発) 木質バイオマスからの高効率バイオエタノール生産システムの研究開発
公開日2012/12/21
報告書年度2011-2011
委託先名国立大学法人京都大学
プロジェクト番号P07015
部署名新エネルギー部
和文要約和文要約等以下本編抜粋。平成23年度では、各プロセスの検討、最適化を行うとともに、ベンチプラントの運転を行った。
英文要約Title: Development of Elemental Technology for Bioenergy Conversion: High Efficiency Bioenergy Conversion Project. Development of Bioethanol Production Process from Woody Biomass Based on High Efficient Solvolysis and Ethanologenic Bacteria (FY2011)
In this project, a new pretreatment system applicable to a wide range of biomass including a fast-growing wood species, Eucalyptus globulus is being developed using microwave irradiation. Novel ethanologenic bacteria capable of producing ethanol directly from woody biomass at a low cost and in a more energy-efficient manner are also being developed. Overall process producing bioethanol by the pretreatment and SSCF (Simultaneous Saccharification and Co-Fermentation) is being developed. In FY2010-2011, a bench-scale plant for bioethanol production was constructed, and improved to apply the SSCF process. Woody biomass was pretreated by a continuous microwave reactor at a rate of 60 Kg wood/h, and converted to bioethanol in the bench-scale plant. The ethanol was distilled and evaluated as a transportation fuel.
In FY2011, microwave reactions in aqueous media were developed for pretreatments of woody biomass, and their reaction conditions were optimized. For enzymatic saccharification, cocktails of cellulolytic and hemiccllulolytic enzymes were screened to increase the efficiency of enzymatic saccharification. At the same time, additives decreasing non-productive binding of cellulase on pretreated biomass were applied. To reduce the cost for pretreatment system, a new microwave reactor unit using 1 kw magnetron for an electric cooker was developed in FY2011. The research group of Tottori Univ. has been breeding novel ethanologenic bacteria capable of producing ethanol directly from woody biomass at a low cost and in a more energy-efficient manner. In FY2011, SSCF process of eucalyptus chips by genetically engineered ethanologenic bacteria, Zb. palmae and Zm. mobilis has been developed.
1) Development of high performance fermentation process: Zm. mobilis BC01 strain was newly isolated as the most suitable strain for SSCF. The strain exhibiting tolerance to higher concentration of sugars could ferment under SSCF condition (at over 35°C, at lower pH5.0).
2) Breeding of ethanolgenic bacteria capable of saccharifying celloologosaccharides and cellulose: Zm. mobilis BC01 strain capable of producing ethanol from cellooligosaccharides was bred by introduction of bacterial ~-glucosidase gene. The engineered strain performed SSF of MW-pretreated eucalyptus pulp.
3) Breeding of ethanologenic bacteria for SSCF(Simultaneous Saccharification and Co-Fermentation) of lignocellulosic biomass: Zm. mobilis BC01 carrying genes encoding xylose catabolic enzymes was suitable to co-ferment a black liquor containing xylose from hemicellulose.
4) Optimization of SSCF process: The genetically engineered Zm. mobilis BC01 exhibited high performance SSCF from MW-pretreated eucalyptus mash in a lab scale.
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