成果報告書詳細
管理番号20130000000427
タイトル*平成24年度中間年報 「次世代機能代替技術の研究開発 次世代再生医療技術の研究開発 少量の細胞により生体内で自己組織の再生を促す自律成熟型再生デバイスの開発(生体内で自律的に成熟する臓器再生デバイスのための基盤研究開発)」
公開日2014/6/14
報告書年度2012 - 2012
委託先名国立大学法人東京大学 国立大学法人大阪大学 学校法人東京理科大学 野村ユニソン株式会社 国立大学法人神戸大学 学校法人福田学園
プロジェクト番号P10004
部署名バイオテクノロジー・医療技術部
和文要約
英文要約Title: Fundamental Research Project for Autonomous Regeneration Device (FY2010-FY2014) FY2012 Annual Report
Reconstruction of joints, based on autonomous regeneration of cartilages, would enable to promote generalization and industrialization of cartilage tissue engineering. In this project, we will establish a fundamental technology to apply the autonomous regeneration for reconstruction of joints. This financial year, for the development of a cell culture technique for an autonomous regeneration device for cartilages, by applying the sustained release system using the hydrogel with growth factors and minute particles, the accumulation of matrices increased by 20 times compared to the conventional transplantation method using atelocollagen. Further, this novel sustained release system enabled low cell density at 106 cells/mL at transplantation; the conventional cell density had been 108 cells/mL. On bones, we examined the mesh size and design of titan shell, and succeeded in finding their optimal requirements. Also, we applied the titan shell to a joint defect model in pig for the fixation of implantation site and confirmed its efficacy. For joints, in culture of mesenchymal stem cells derived from porcine synovium, we established the serum-free culture method using artificial medium. Further, we verified that 3D artificial tissues could be produced by using porcine cells with serum free medium. As the production of hollow fibers made of biodegradable polymers, using biodegradable polymer and poly lactic acid, we developed the hollow fiber membrane, considering the requirements of production and composition. The permeability of the membrane produced with a metal cap (external diameter was 1.5 mm) was 880 to 950 L/(m2 h atm). Regarding hydrogel, we optimized the condition of gelation system by chemical crosslinking, and succeeded in developing the novel gel, which held the 3D structure of peptide fibers and kept the mechanical strength. As exploratory animal experiment for cartilage, we carried on transplantation experiments on pigs, using mesenchymal stem cells obtained from bone marrow, and developed a cartilage and bone defect model by forming cartilage and bone defects on articular surfaces of porcine tibia. Applying this model, we transplanted porcine mesenchymal stem cells obtained from bone marrow to the defect site. As a result, we observed the better cartilage regeneration with the cells than without cells, and also succeeded in decreasing the cell density to 107 cells/mL at transplantation.
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