成果報告書詳細
管理番号20130000000501
タイトル*平成24年度中間年報 「次世代機能代替技術の研究開発 次世代再生医療技術の研究開発 少量の細胞により生体内で自己組織の再生を促す自律成熟型再生デバイスの開発(Muse 細胞を用いたin situ stem cell therapyの基盤研究開発 実用化研究開発)」
公開日2014/6/11
報告書年度2012 - 2012
委託先名国立大学法人東北大学 国立大学法人京都大学 株式会社Clio 国立大学法人名古屋大学
プロジェクト番号P10004
部署名バイオテクノロジー・医療技術部
和文要約
英文要約Title;Research and Development of Next-generation Regenerative Technology / Research and development of in situ stem cell therapy using Muse cells (FY2010-FY2014) FY2012 Annual Report
This project clarifies the findings that form the base of in situ stem cell therapy using Muse cells, and aims to establish highly effective and safe next-generation cell therapy technology linked to in vivo autonomous tissue regeneration/repair and function recovery.
1. Research and development of introduction of Muse cells into lesions
1-1 We generate model mice with liver degeneration and examine the substances in their peripheral blood.
1-2 We have deciphered the mechanism of tissue repair for a certain organism and identified a migration factor and migration inhibitory factor. In addition, we have shown that the migration factor promotes migration of Muse cells.
1-3 Using the results of the proteome and expression analyses of non-Muse and Muse cells, we have made a list of membrane proteins characteristic of Muse cells. Among those proteins, we have confirmed that Muse cells specifically express the migratory factor candidates identified in 1-2. In addition, we have examined other migratory factor candidates.
1-4 We have launched an analysis system using TAXIScan to observe in real time if the migratory factor candidates identified in 1-2 promote the migration of Muse cells.
2. Research and development on engrafting Muse cells at lesion sites
Based on the information from literature to date, we have preliminarily examined several kinds of material suitable for the scaffold to promote the engraftment and differentiation of Muse cells.
3. Research and development on migration and regulation of differentiation of Muse cells
3-1 For the induction of in vivo migration, we have examined the migratory factor candidates identified in 1 using mice and verified that those candidates induce the migration of Muse cells in vivo.
3-2 In our basic research on regulation of differentiation, we have succeeded in the induction of Muse cell differentiation into melanocyte (a pigment cell). On the other hand, we have shown that xeno-Muse cells barely induce immune responses.
3-3 For skin diseases, we have succeeded in inducing the differentiation of Muse cells into melanocytes, and prepared a 3-D cultured skin using the melanocytes. We grafted the cultured skin onto mice and verified that the melanocytes from Muse cells function in the skin.
3-4 For neurodegenerative diseases, we have developed an experimental system using model mice/rats with cerebral infarction, and have grafted human undifferentiated Muse cells into the animals’ brains to examine the regenerative effect of Muse cells.
4. Survey Research for commercialization
For the practical use of the findings of this project, we have negotiated a tie-up with potential co-developers. In addition, we have reviewed the basic patent for Muse cells which is a prerequisite to this project. As a result, we have succeeded in the development of the main part (substance patent and isolation method of Muse cells) of the basic patent in Japan.
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