成果報告書詳細
管理番号20130000000696
タイトル*平成23年度中間年報 「次世代機能代替技術の研究開発 次世代再生医療技術の研究開発 少量の細胞により生体内で自己組織の再生を促す自律成熟型再生デバイスの開発(Muse細胞を用いたin situ stem cell therapyの基盤研究開発 実用化研究開発)」
公開日2014/6/11
報告書年度2011 - 2011
委託先名国立大学法人東北大学 国立大学法人京都大学 株式会社Clio
プロジェクト番号P10004
部署名バイオテクノロジー・医療技術部
和文要約
英文要約Research and Development of Next-generation Regenerative Technology / Research and development of in situ stem cell therapy using Muse cells (FY2010-FY2012) FY2011 Annual Report
This project clarifies the findings that form the base of in situ stem cell therapy using Muse cells, and aims to establish highly effective and safe next-generation cell therapy technology linked to in vivo autonomous tissue regeneration/repair and function recovery.
1. Research and development of induction of Muse cells into lesions
1-1 We have generated a mouse model of degenerative liver diseases and established an experimental system to collect cells and substances from peripheral blood of the mice.
1-2 We have deciphered the mechanism of tissue repair for a certain organism and identified a migration factor and migration inhibitory factor. In addition, we have shown that the migration factor promotes migration of Muse cells.
1-3 We have made a list of membrane proteins characteristic of Muse cells employing the results of proteomic analysis of Muse and non-Muse cells performed by Prof. Toshiaki Isobe of Tokyo Metropolitan University. Most importantly, we have confirmed that the receptor of the migration factor candidate that we identified in 1-2 is expressed in Muse cells only.
1-4 We have established a system to confirm the migration status of Muse cells with an electron microscope and have demonstrated the analysis system using TAXIScan.
2. Research and development on engrafting Muse cells at lesion sites
Based on an extensive review of literature last year, we have performed
some preliminary experiments to examine materials that might provide a suitable foothold for Muse cell engrafting and differentiation enhancing.
3. Research and Development on differentiation control in vivo
3-1 In our research on in vivo migration of Muse cells, we have examined the preparation of the slow release of the migration factor candidate identified in 1 and have addressed mouse-based experimental systems.
3-2 In our basic research on regulation of differentiation, we have succeeded in the induction of Muse cell differentiation into melanocyte (a pigment cell). In addition, we are also examining the immune responses to allogeneic Muse cells or cells differentiated from allogeneic Muse cells.
3-3 In our research on skin diseases, we have succeeded in the preparation of three-dimensional cultured skin by using melanocytes that successfully differentiated from Muse cells. We have also established an experimental system using a mouse model of cerebral infarction in relation to neurodegenerative diseases.
4. Survey Research for commercialization
We have modified the patent applications (2 applications) in association with the projects for which we applied last year by adding new experimental results.
In addition, we have made a list of companies with which we are potentially able to collaborate in research for commercialization using the results of this project.
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