成果報告書詳細
管理番号20130000001033
タイトル*平成24年度中間年報 「次世代機能代替技術の研究開発 次世代再生医療技術の研究開発 生体内で自己組織の再生を促すセルフリー型再生デバイスの開発 幹細胞ニッチ制御による自己組織再生型心血管デバイスの実用化開発」
公開日2014/6/11
報告書年度2012 - 2012
委託先名ニプロ株式会社
プロジェクト番号P10004
部署名バイオテクノロジー・医療技術部
和文要約
英文要約Title:Research and Development of Next-generation Regenerative Technology. Development of cell-free regenerative device which promotes regeneration of autologous tissue in vivo. Development of self-regenerating cardiovascular device by regulating stem cell niche. Annual Report FY2011(period: FY2010-FY2012).
(1) Study of material selection and processing (Making biodegradable cardiac support device)
We studied that making and processing of customized cardiac support device from a human cardiac 3D-data. In result we have been able to make customized cardiac support device that fit the heart ventricular shape at suitable pressure.
We made trial of various processing ways such as knitting and depositing with various materials. As a result we found that dedicated machine was necessary for making customized cardiac support device and that poly-glycolic acid was one of the suitable materials for making it with the machine. Poly-glycolic acid is biodegradable material. Poly-glycolic acid yarn has enough strength for knitting with the machine and has shown good result on clinical use.
(2) Study of manufacturing (assembling) technology
As above we found that we can make customized cardiac support device with dedicated machine. We next studied coating technology to release factors from cardiac support device.
(3) Making device for evaluation (Making device for animal experiment)
As above we found that we can make customized cardiac support device of poly-glycolic acid. In the next step, we made cardiac support device that were suitable for the size of dog heart for animal experiment. The device we made has a slit to ease setting up to dog heart. The experiment is in progress at Osaka University. And we got a good data from dog heart failuredmodel.
(4) In vitro evaluations of device (Biological safety and Coating the tissue regenerative factor to cardiac support device)
We performed a cytotoxicity test as one of the biological safety tests. In result the device had no toxicity (IC50 value: 100%).
Coating the stem cell inducing/differentiating factor to cardiac support device was performed with gelatin-stem cell inducing/differentiating factor mixture. We confirmed the coated surface of cardiac support device with electro-microscope and there were “stem cell inducing/differentiating factor” on the surface of cardiac support device with gelatin layer. And we have discovered a new type of drug delivery system in two layered gelatin films. One film is including “stem cell inducing /differentiating factor” in good dispersed. The other film is cover layer to slow release the “stem cell inducing /differentiating factor” from above one.
(5) Optimizing a manufacturing process of device
As above we found that we try to any way to get a optimized device manufacturing process in good reproduction. We will make discovery a good manufacturing process in computer controlled knitting machine.
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