成果報告書詳細
管理番号20130000000275
タイトル*平成24年度中間年報 「ヒト幹細胞産業応用促進基盤技術開発 ヒト幹細胞実用化に向けた評価基盤技術の開発」 (浜松ホトニクス株式会社 )
公開日2014/8/20
報告書年度2012 - 2012
委託先名浜松ホトニクス株式会社
プロジェクト番号P10027
部署名バイオテクノロジー・医療技術部
和文要約
英文要約The aim of this NEDO (New Energy and Industrial Technology Development Organization) Project is the “Development of Core Technologies for Industrial Applications of Human Stem Cells”. In recent years, great progress has been made worldwide in developing drug discovery screening and cell therapy applications for hPSCs (human pluripotent stem cells, such as hES cells).However, large scale PSC culture methods vary widely across the globe, and are in need of standardization. Initiated last year in Japan, this six-year national project supported by METI (the Ministry of Economy, Trade and Industry) aims to develop not only large scale production methods, but also the technologies necessary for stem cell evaluation.
1. Development of robust, mass culture and cryo-preservation technologies for hPSC lines
(4) Multi-parameter imaging technology for living cell in the culturing.
When we make human hES cells proliferate to obtain a large amount of hES cells with
high quality, the continuous monitoring of the quality of the cells in culture with
non-invasive manner is needed. We have been developing a novel optical microscope-based
method to evaluate the quality of cultured cells continuously using various morphological
and optical parameters of the cells in culture. In this fiscal year, we constructed the prototype of the microscope-based imaging system that obtains to obtain various optical parameters of living cells in culture. Using this microscope system, we measured the optical parameters, which is thought to reflect the structural features of the cells, of various differentiated adult cells such as fibroblasts, hepatocytes, brown adipocytes, and microvascular endothelial cells. When compared the optical parameters in various cell types, we found that each cell type shows the different distinctive characteristic in the optical parameters. Next, we measured optical parameters of hES cells in the good and the bad culture conditions. We found the characteristic pattern of optical parameters that distinguishes between cells in the good and in the bad culture conditions. Our results suggest that the optical parameter, which can be obtained with non-invasive manner, is one of the reliable indexes to evaluate the quality of hES cells.
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