成果報告書詳細
管理番号20140000000448
タイトル*平成25年度中間年報 「がん超早期診断・治療機器の総合研究開発 超早期高精度診断システムの研究開発:血液中のがん分子・遺伝子診断を実現するための技術・システムの研究開発 血中分子・遺伝子診断のための基礎技術の研究開発(癌マーカー細胞群の濃縮・検出・培養技術の確立とその診断応用)」
公開日2015/3/24
報告書年度2013 - 2013
委託先名株式会社オンチップ・バイオテクノロジーズ
プロジェクト番号P10003
部署名バイオテクノロジー・医療技術部
和文要約
英文要約The objective of the project is to achieve optimal cancer treatment in patients with the precise diagnosis of cancer such as early detection of metastasis and/or malignant grade by blood tests. The team is developing the protocol and its diagnostic equipment which are to detect and sort CTCs by disposable microfluidics chip based flowcytometer, after concentrate CTCs from peripheral blood.

The achievements of FY2013 are as follows,
We have established immunocytochemical staining for vimentin to detect CTCs that underwent EMT and we were successful in detection and isolation of EpCAM-negative and cytokeratin-negative CTCs using clinical samples.
Somatic mutations in cancer-related driver genes were successfully detected using CTCs isolated from patients with lung cancer and breast cancer. Of note, those mutations were detected in patients who were CTC-negative by CellSearch method.
In clinical studies conducted this year, driver mutations were successfully detected using CTCs after whole genome amplification by next generation sequencing and this implies the potential of comprehensive mutational analysis using in cancer-related genes using CTCs.
The equipment and micrfluidic chip has been improved and optimized for more sensitive and precise detection of CTCs.
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