成果報告書詳細
管理番号20150000000504
タイトル*平成26年度中間年報 バイオマスエネルギー技術研究開発 バイオ燃料製造の有用要素技術開発事業 バイオ燃料事業化に向けた革新的糖化酵素工業生産菌の創製と糖化酵素の生産技術開発
公開日2015/9/1
報告書年度2014 - 2014
委託先名花王株式会社 国立大学法人長岡技術科学大学 一般財団法人バイオインダストリー協会
プロジェクト番号P13011
部署名新エネルギー部
和文要約
英文要約Title: Construction of Innovative Saccharifying Enzyme-producing Microorganism and Development of Manufacturing Technology of the Enzyme for the Biofuel Commercialization (FY2013-FY2016) FY2014 Annual Report

This project is aimed at the development of a process technology package that can provide enzymes for bio-ethanol production on a commercial scale by 2020. To achieve the goal of the project, we worked on three research subjects
1)Development of high performance saccharification enzymes
  We are trying to establish an analysis method and the ideal culture conditions for improved methods of searching for high-performance enzymes. To acquire high-performance enzymes, we have searched for candidate genes among various kinds of microorganisms and we modified the major enzymes for heat and glucose resistance by gene mutagenesis. As a result, we have cloned thirty genes and discovered that one enzyme of the selection genes improves the saccharification performance.
2) Engineering of industrial production strains optimized for the production of saccharification enzymes
  Cellulase-overproducing mutants of Trichoderma reesei are considered as the prime source of enzymes needed for the economic saccharification of pretreated cellulosic biomass. From the viewpoint of simultaneous protein productivity, we selected the PCD-10 strain in FY2013 as the host for future activities. However, PCD-10 provided an unsatisfactory cellulase production volume. Therefore, we tried to perform mutation breeding of T. reesei for cellulase overproducing mutants. As a result, we selected one mutant that produces cellulase in media containing powdered cellulose under conditions of higher glucose concentrations. In addition, we found that cellulase production by this mutant increased 1.2-fold, compared to that by PCD-10.
3)Development of low-cost large-scale production of saccharification enzymes using T. reesei
  The use of low-cost substrates such as herbaceous biomass that has undergone alkaline pretreatment was suggested as an alternative to reduce production costs. The fermentation parameters (product and cells) of T. reesei were investigated by utilizing data on cell growth, but the commercial production of the technology that has been developed in the laboratory for industrial application has to be upscaled from the bench scale to the industrial scale. In addition, the post-processing MF and UF steps were examined after the culture. We had built several kiloliter-scale fermentation pilot plants.
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