成果報告書詳細
管理番号20130000000866
タイトル*平成24年度中間年報 バイオマスエネルギー技術研究開発 戦略的次世代バイオマスエネルギー利用技術開発事業 海洋性緑藻による油脂生産技術の研究開発
公開日2015/11/20
報告書年度2012 - 2012
委託先名国立大学法人神戸大学 大学共同利用機関法人自然科学研究機構基礎生物学研究所 DIC株式会社
プロジェクト番号P10010
部署名新エネルギー部
和文要約
英文要約Title: Development of Oil Production Process Using Marine Green Algae (FY2012-FY2013) FY2012 Annual Report

A.Kobe University
Marine green alga Chlamydomonas orbicularis has not only high growth ability but high oil content. C. orbicularis is useful for the development of mass cultivation system and genomic transformation technique because of close relationship with model alga C. reinhardtii. Algal oil production by marine-water cultivation has advantages in elimination of fresh water and nutrition, reduction of infrastructure costs and energy saving because of the usage of marine water. Novel cultivation system is developed to maximize oil productivity by realizing both high cell density culture and high oil content in the cell. The oceanic microalgae of C. orbicularis gave the relatively high growth rate of more than 450 mg /L/d and high lipid productivity of more than 130 mg/L/d under 3.5% artificial sea water, which is superior than most of the related reports. Lipid production by oceanic microalgae of C. orbicularis gave huge potential as the promising oil producer due to its high sanity tolerance, high cell growth rate, and high lipid productivity.
B.National Institute of Basic Biology
(1) The growth of the oil-producing green alga Chlamydomonas orbicularis Tai-04
We found the green alga Chlamydomonas orbicularis Tai-04 can be grown photoheterotrophically in the presence of acetate much as like the model species Chlamydomonas reinhardtii. Furthermore, this oil-producing green alga is not halophilic, but halotolerant alga that can be grown in fresh water media. We tested the effective concentrations of antibiotics paromomycin and spectinomycin at several different concentrations in a fresh water and sea water media, and found that they effectively killed C. orbicularis Tai-04 in the former, but not in the latter media, suggesting the possibility of screening the transformants by these antibiotics.
(2) Methods for transforming the oil-producing green alga Chlamydomonas orbicularis Tai-04
Because we expected that the presence of cell wall could be a problem when we introduce exogenous DNA into the nuclear genome of C. orbicularis Tai-04 as known in the model alga C. reinhardtii, we examined several different methods for introducing DNA into Chlamydomonas cells. We found the pulsed electroporation and particle-gun delivery were effective in the nuclear and chloroplast genome, respectively, in the model species.
C.DIC Corporation
(1) Laboratory culture
Laboratory culture of Chlamydomonas orbicularis Tai-04 strain that will be used as seed culture for outdoor experiment has been established. Standard cultures were grown in HSM medium, under continuously lighting (150 μmol/m2/s) and air containing 1% CO2. Favorable growth were obtained under wide range of temperature between 25℃ and 35℃, and wide range of pH between 7 and 10. Scale up culture using 20L plastic bottle showed 200mg/L/d of highly biomass productivity.
(2) Set up of outdoor culture facility
Outdoor culture facility was set up at Earthrise Nutritionals LLC which is a manufacturer of blue green alga Spirulina, subsidiary company of DIC Corporation located in California, United State. We designed and installed 1.1 m2, 2.7 m2 and 50 m2 race way ponds. Those have been confirmed ready to use by test run, mixing speed measurement, and so on.
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