成果報告書詳細
管理番号20120000000170
タイトル*平成23年度中間年報 新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発 (先導技術開発) イオン液体を利用したバイオマスからのバイオ燃料生産技術の開発
公開日2015/12/4
報告書年度2011 - 2011
委託先名国立大学法人神戸大学 株式会社豊田中央研究所 国立大学法人九州大学 トヨタ自動車株式会社
プロジェクト番号P07015
部署名新エネルギー部
和文要約
英文要約Title:Development of Bio-fuel production technology by ionic liquid (FY2009-FY2012) FY2011 Annual Report

 In this study, for deconstruction of biomass, the ionic liquid was used as a pre-treatment medium, and the effective degradation and assimilation procedure was investigated. This new and effective ethanol production process has been named as 'Ionic liquid-based Consolidated Bio-Processing (i-CBP)', and we make a consortium with 4 research institutes as follows:
(1) Development of ethanol fermentation process by one batch operation (arming yeast technology)
Using four cellulases displayed yeast strain, the ethanol fermentation from ionic liquid pre-treated bagasse was performed. As a result, the ethanol yield without any additional cellulase reagents was attained 50 % of theoretical yield. Meanwhile, the complete degradation and conversion to ethanol was attained with additional enzyme. In addition, the construction of cellulases displayed arming yeast optimized for ionic liquid pre-treated bagasse was also performed, and we could find out the optimized arming yeast. By using this new strain, we will plan to asses an ethanol production potential from ionic liquid pre-treated bagasse in next year.
(2) Development of ethanol fermentation process with two batch operation.
To develop the low cost of biomass pretreatment process, we examined the scale up using the diluted ionic liquid with an organic solvent (mg to g scale). Even if it was an ionic liquid diluted to twice with the solvent, both celluloses and hemicelluloses were able to confirm high saccharification efficiency. Subsequently, we started to develop the transgenic yeast strain that was secretary produced cellulase with optimal ratio, and examined the ethanol fermentation from ionic liquid pre-treated biomass with two batch operation.
(3) Design and selection of ionic liquids for effective biomass pretreatment.
The potential of a pyridinium ionic liquid (PyIL) to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose and bagasse at 100 °C. We found that the PyIL pretreatment for an extended duration released mono- and disaccharides without using cellulases, suggesting the capability for direct saccharification of cellulosic feedstocks. It was also found that a PyIL pretreatment enhanced initial saccharification rates by partial degradation of the crystalline cellulosic fraction in biomass rather than the delignification.
(4) Development of ionic liquid recovery and recycle.
 In the case of two batch process, we examined the recovery and recycling of diluted ionic liquid with an organic solvent. By evaluating of 50 times with recycled ionic liquid, it was confirmed that there was no influence in the saccharification efficiency even if the diluted ionic liquid. Now, we are examining the effect of the kind and the grain diameter of the biomass in the enzymatic degradation efficiency.
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