成果報告書詳細
管理番号20160000000152
タイトル*平成27年度中間年報 バイオマスエネルギー技術研究開発/戦略的次世代バイオマスエネルギー利用技術開発事業(次世代技術開発)/高油脂生産微細藻類の大規模培養と回収および燃料化に関する研究開発
公開日2016/5/28
報告書年度2015 - 2015
委託先名株式会社デンソー 学校法人中央大学 株式会社クボタ 出光興産株式会社
プロジェクト番号P10010
部署名新エネルギー部
和文要約
英文要約R&D on Mass Cultivation and Harvesting of Microalgae with high oil content and Hydrotreating Process for biofuel production.

Summary
Microalgal-derived biofuel is recognized as a third generation biofuel, because it does not compete with food and has a high potential for accumulating oil. We have to develop i) energy-saving and automatic cultivation system, ii) harvesting processes, and iii) catalyst technology for producing drop-in fuel toward the practical use, iv) improving microalgal oil production ability by gene manipulation such as self-cloning. We have some plans to reduce cultivation costs to a practical level, such as improving water recycle technologies, developing automation system and reducing the power consumption required for the culture. We had studied how to remove the organic matter in a broth and find good porous carrier for microbial degradation. This microbial carrier improve the decomposition rate of the organic matter by 2.5 times than Sirasu. We also find that protozoa contamination were reduced by lowering the dissolved oxygen concentration of the pond during the night. Combination of lowering dissolved oxygen and adding chlorine disinfectant in a pond make it possible to extend the culture period to more than 2 months for the outdoor continuous cultivation of pseudococcomyxa sp. Membrane filtration system have been used for the harvesting of cultured algae. This year the reactor configuration was first optimized to establish higher recovery rate, higher concentration rate and lower operation cost. We have tested three types of reactors: 60 slope type, V shape type and high density tube type. Among these reactors, the V types showed the best performance in terms of recovery rate, concentration rate and fouling prevention. We also investigated the performance of membrane harvesting process when the pre- and main-cultivations were carried out in outside. As a result of 13 times of cultivation-harvesting tests, all the culture runs filed to operate the membrane harvesting process under high flux because of high fouling tendency. Based on the detailed analysis of the culture solution and fouling characteristics, the following fouling mechanisms was suggested. To achieve more efficient operation, we have suggested some new systems including (1) two-step harvesting process, (2) pressurized membrane filtration system or (3) cyclone-membrane hybrid systems. Next year we are plan to investigate the performance of these systems. We have been developing algal oil purification technology as a pre-treatment for the production of hydro-treated bio-jet (HBJ) and studying a design of bio-jet fuel. We had developed the gene manipulation technology such as gene editing (TALEN and CRISPR ), promoter / terminator systems which give high level of expression in nitrogen deficient cell of pseudococcomyxa sp, efficient selection methods for high expression transgene cell in order to realize high oil productivity pseudococcomyxa strains.
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