成果報告書詳細
管理番号20160000000154
タイトル*平成27年度中間年報 バイオマスエネルギー技術研究開発/戦略的次世代バイオマスエネルギー利用技術開発事業(次世代技術開発)/微細藻類の改良による高速培養と藻体濃縮の一体化方法の研究開発
公開日2016/5/28
報告書年度2015 - 2015
委託先名株式会社IHI 国立大学法人神戸大学 株式会社ちとせ研究所
プロジェクト番号P10010
部署名新エネルギー部
和文要約
英文要約Title: Development of integrated method for high speed cultivation and algal cell concentration (FY2012-FY2015) FY2015Annual Report

Hyper-Growth Botryococcus braunii, HGBb, is phototrophic algae strain improved without using genetic recombination technique, and characterized by unprecedentedly high speed in growth rate and high percentage of hydrocarbon oil concentration.

Our four-year-long research and development project has five objectives. The first one is to establish low-cost cultivation technique in an open environment using sunlight as a sole source of energy, and integrate it into harvesting, concentrating, and oil-extraction process. The second is to reduce the overall operational costs by adding useful features to the strain (improved HGBb). The third is to develop the gene insertion methodology and apply it to HGBb cells for the genetic recombination. The forth is to explore sites for commercial production while conducting cultivation tests at various parts of the world and checking its growth rate and adaptability to local climate. And the last is to stably cultivate improved HGBb in 1500 m2 open pond.

We have made progresses in achieving these objectives this year. We accomplished developing cell concentration method by reducing water content to 90% by weight by taking advantage of improved HGBb’s floating feature. Moreover, we have confirmed the algae’s productivity in the open pond as well as in closed container in the past year using exhaust gas from coal-fired power plant, which is one of the cheapest source of CO2. We have determined conditions for genetic recombination using a particle gun and electroporation method to insert the plasmid, however, genes introduced into inner cell is not yet identified. Therefore, we have tried to detect the mRNA. To explore the commercial cultivation site, we carried out small cultivation test (~5 m2) in south-east Asia (2 sites) and U.S.A., where the amount of solar radiation is higher than that of Japan. The productivity of improved HGBb recorded over 20 g-dry weight/(m2・d) in foreign test sites. We succeed in stable cultivation using integrated process from cultivate to harvest to concentrate consistency at the pilot plant (cultivation area: 1500 m2, open pond) in Kagoshima, Japan. The productivity and oil-contents of improved HGBb are over 9 g-dry weight/(m2・d) and around 50%, respectively.
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