成果報告書詳細
管理番号20160000000312
タイトル*平成27年度中間年報 バイオマスエネルギー技術研究開発 バイオ燃料製造の有用要素技術開発事業 有用微生物を用いた発酵生産技術の研究開発
公開日2016/6/1
報告書年度2015 - 2015
委託先名日揮株式会社 国立研究開発法人産業技術総合研究所 学校法人君が淵学園崇城大学 一般財団法人バイオインダストリー協会
プロジェクト番号P13011
部署名新エネルギー部
和文要約
英文要約Title: Bio-Energy Technology Development, Project on the Development of Useful Elemental Technology for Biofuel Production, Development of a fermentation technology using the microorganism (FY2013-FY2016) FY2015 Annual Report

In this project, C5C6 simultaneous saccharification and co-fermentation (SSCF) organism and process will be developed, process design package will be prepared, and economic evaluation will be conducted.

1) Construction of C5C6 co-fermenting microorganisms.
The aim of this study is to construct the efficient ethanologenic yeast and to analyze the genetic information of the strain leading to potent xylose utilization, thermotolerance and fermentation-inhibitor tolerance in the SSCF process. Four thermotolerant mutants and three xylose utilizing mutants of Saccharomyces cerevisiae had been isolated in FY2014. In the FY2015, three fermentation-inhibitor tolerant mutants were isolated. In addition, xylose reductase, xylitol dehydrogenase, and xylulose kinase of thermotolerant yeast Kluyveromyces marxianus DMB1 were analyzed.
Comprehensive genome analysis of xylose utilization and thermotolerance mutant strains were leading to identification of xylose utilization and thermotolerant genes. Furthermore, 36 of genetically engineered strains were examined by established standardized evaluation SSCF methods. B16 strain showed the highest performance during SSCF of NaOH-treated bagasse. B4 and B6 strains showed the highest yield during SSCF of diluted acid treated bagasse.

2) Development of an efficient SSCF process
SSCF of eucalyptus pulp, NaOH-treated bagasse and diluted acid-treated bagasse using genetically modified S. cerevisiae were tried in 30L or 200L scale SSCF reactors. In SSCF of eucalyptus pulp using 200L reactor, project targets (ethanol concentration and yield) were achieved.
Slurry handling tests were carried out by 1m3 scale slurry pump apparatus and 2m3 scale slurry handling apparatus to obtain data for high concentration slurry handling and designing the pilot scale SSCF reactor. The 2m3-SSCF reactor for genetically engineered yeasts was constructed based on the data of slurry handling tests. 2m3-SSCF trials have been started.
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