成果報告書詳細
管理番号20160000000337
タイトル*平成27年度中間年報 エネルギー・環境新技術先導プログラム 生物・有機合成ハイブリッド微生物による100%グリーンジェット燃料生産技術の開発
公開日2016/6/22
報告書年度2015 - 2015
委託先名公益財団法人地球環境産業技術研究機構
プロジェクト番号P14004
部署名イノベーション推進部
和文要約
英文要約Title: Advanced Research Program for Energy and Environmental Technologies / Development of Production Technology for 100% Green Jet Fuel by Hybrid Microbe Equipped with Chemical Catalysts (FY2014-FY2016) FY2015 Annual Report

In our project, we are planning to create a hybrid microbe by introducing unnatural chemical reactions exclusively used in chemical synthesis into a microbe, which enable to produce unnatural hydrocarbon derivatives with various backbones by fermentation.
Hydrocarbon derivatives produced by our hybrid microbe would be easily converted to branch- and cyclo- hydrocarbons and aromatics suitable for jet fuels.
This project consists of four sections: “Functional expression of chemical catalysts in microbe”, “Screening of tailoring enzymes”, “Development of a novel cofactor-regeneration system coupled with generation of hydrogen”, and “Construction of a hybrid microbe equipped with chemical catalysts. In fiscal 2015, we examined the former three sections.
The first section aimed at feasibility demonstration of functional expression of chemical catalysts in microbe. Chemical catalysts which are unnatural synthetic compounds were expressed in the form of peptides as their mimics in microbe. Chemical catalysts which are natural compounds were overproduced by introducing their biosynthetic pathways in microbe. Catalytic activities of recombinant strains accumulating each chemical catalyst in the cytoplasm were screened by cell-based assay. As a result, two of peptide mimics and two of natural compounds were successfully expressed as active catalysts within the microbe.
The second section aimed at acquisition of tailoring enzymes which could convert intermediate compounds produced by chemical catalysts to hydrocarbon derivatives. Various kinds of enzymes derived from bacteria and fungi were screened for activity, however, none of these enzymes were active on the desired intermediate compounds. Instead, it was found that the chemical catalysts successfully expressed in microbe in the first section possessed the desired activity as well.
The third section aimed at heterologous expression of hydrogenases in Corynebacterium glutamicum. Sets of genes required for heterologous expression of hydrogenases were cloned, and then introduced in C. glutamicum. By western blotting detection method, it was successfully confirmed that hydrogenases were expressed in a soluble form.
The fourth section aims at production of hydrocarbon derivatives from glucose. Construction of hybrid microbe is proceeding.
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