成果報告書詳細
管理番号20160000000498
タイトル*平成27年度中間年報 バイオマスエネルギー技術研究開発 バイオ燃料製造の有用要素技術開発事業 バイオ燃料事業化に向けた革新的糖化酵素工業生産菌の創製と糖化酵素の生産技術開発
公開日2016/6/22
報告書年度2015 - 2015
委託先名花王株式会社 国立大学法人長岡技術科学大学 一般財団法人バイオインダストリー協会
プロジェクト番号
部署名新エネルギー部
和文要約
英文要約Title: Construction of Innovative Saccharifying Enzyme-producing Microorganisms and Development of Enzyme Manufacturing Technology for Biofuel Commercialization (FY2013-FY2016) FY2015 Annual Report

This project is aimed at the development of a process technology package that can provide enzymes for bio-ethanol production on a commercial scale by 2020. To achieve this goal, we worked on three research subjects:
1) Development of high-performance saccharification enzymes
    We are trying to establish an analysis method and ideal culture conditions for improved methods to identify high-performance enzymes. To acquire such enzymes, we have searched for candidate genes from various types of microorganisms and have modified the major enzymes for heat and glucose resistance by gene mutagenesis. As a result, we cloned 25 genes and discovered that several enzymes (based on gene selection) improve saccharification performance.
2) Engineering of industrial production strains optimized for the production of saccharification enzymes
    Cellulase-overproducing mutants of Trichoderma reesei are considered the prime source of enzymes required for the economic saccharification of pretreated cellulosic biomass. From the viewpoint of simultaneous protein productivity, we selected cellulase-overproducing mutants in FY2014 for future activities. Genomic analysis of the mutant strain was performed to identify the mutation.
By comparative genomic analysis, single nucleotide polymorphisms (SNPs) in each strain and the gene defect (DEL) were analyzed. As a result, we confirmed that the effect on protein productivity was due to one gene. In addition, we constructed a new industrial production strain, which expressed BGL and Kao XYN. As a result, high production of high-performance enzymes is possible.
3) Development of low-cost, large-scale production of saccharification enzymes using T. reesei
    The use of low-cost substrates such as herbaceous biomass that has undergone alkaline pretreatment was suggested as an alternative to reduce production costs. The fermentation parameters (product and cells) of the new industrial production strain were investigated by utilizing data on cell growth. However, the commercial production of technology that has been developed in the laboratory for industrial application needs to be up-scaled from the bench- to the industrial scale. In addition, we cultured the first industrial production strain, JN, using several kiloliter-scale fermentation pilot plants, and the post-processing MF and UF steps were examined after culture.
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