成果報告書詳細
管理番号20160000000460
タイトル*平成27年度中間年報 国際研究開発・実証プロジェクト コファンド事業 フランスBpifranceとの国際研究開発・実証事業 蛍光ナノイメージングを用いた創薬支援システムの開発
公開日2016/7/13
報告書年度2015 - 2015
委託先名コニカミノルタ株式会社
プロジェクト番号P14005
部署名ロボット・AI部
和文要約
英文要約Title: Co-fund Project. International R&D and Business Verification Project with Bpifrance.Development of Drug Discovery Support Systems using Fluorescent Nanoparticles Imaging Technology.(FY2015-2017) FY2015 Annual Report.

Based on need of drug discovery, observation subjects necessary for drug discovery are discussed and determined, and fluorescence nanoparticle developed by Konica Minolta, Inc.(KM) is used for the study and development of high-resolution bio-imaging technology of those subjects performed by the most advanced microscopes. In 2015, super-resolution image observing apparatus manufactured by BioAxial was used and resolution-centered basic features came to be known. In specific, KM nanoparticle was used for the verification of resolution, S/N ratio and features of image signals. In addition, to verify that materials and technologies developed by this project meet the needs of drug discovery scenes, observation samples are made to make superfine and local imaging inside cells or quantitative evaluation on molecules such as protein possible, and these samples are observed by image observing apparatuses for new fluorescence materials, technology improvement and comprehensive application of existing apparatus. In 2015, a cell culture laboratory was established, and KM fluorescence nanoparticles became known that is able to appreciate the efficiency of anti-HER2 antibody drug. The target is to apply imaging base technology to the medicine development process that meet specific needs, and to start to perform experimental analyses entrusted by pharmaceutical enterprises. In specific, apparatuses that analyze vital reaction by the imaging of a number of markers on protein and the like at the same time is used to promote development of entrustment preparation. In 2015, external equipment was applied to establish an environment where mice in vivo imaging apparatuses or confocal microscopes can be used. Further, high-sensitive mice in vivo imaging apparatuses were used to a establish simulation system of the FUEL phenomenon using KM fluorescence nanoparticles and results on luminescence extraction features were obtained. Also, high-sensitive plate readers for luminescence assay were introduced, and feature evaluations on fluorescence nanoparticle could be performed. Based on problems drawn from the creation process of bio-imaging base technology, KM designed and tried to produce a new fluorescence nanoparticle that is suitable for observation. When an object deep inside a living body is to be observed, it is considered that visible light may attenuate. Aiming at this problem, fluorescence nanoparticles equal to commercial near-infrared fluorescent dye of indocyanine green were designed and put into trial production. In addition, the company incorporated with the development of fluorescence nanoparticles which has a large Stokes-shift. In 2015, a synthesis of near-infrared fluorescence nanoparticles including indocyanine green was performed and could be used for in vivo experiment. Also, in terms of particles including Stokes-shift dye, the dye cost was high and became a problem in view of business feasibility, so its replacement was discussed.
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