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成果報告書詳細
管理番号20170000000263
タイトル*平成28年度中間年報 植物等の生物を用いた高機能品生産技術の開発 植物の生産性制御に係る共通基盤技術開発 ゲノム編集の国産技術基盤プラットフォームの確立
公開日2017/6/7
報告書年度2016 - 2016
委託先名エディットフォース株式会社 国立大学法人東京医科歯科大学 国立大学法人神戸大学 国立研究開発法人産業技術総合研究所 国立大学法人広島大学 国立研究開発法人理化学研究所 国立大学法人九州大学 国立大学法人東京大学 知的財産戦略ネットワーク株式会社
プロジェクト番号P16009
部署名材料・ナノテクノロジー部
和文要約
英文要約Summary for report 2016
Research & Developmental Program
Development of bio-production technologies of valuable substances using plants
- Development of basic and fundamental technologies for bio-production using plant
- Establishment of Japanese technological platform of genome editing
(Kyushu Univ., Tokyo Medical and Dental Univ., Kobe Univ., Hiroshima Univ., Tokyo Univ., AIST, RIKEN, EditForce Inc., IPSN, Inc.)

Summary: This program has been launched to establish fundamental, independent technological platform of “genome editing” and its use in bio-production area, to improve the international industrial competitiveness for Japan. This program has also aimed the practical use of newly established genome editing technologies for production of valuable substances using plants. To achieve this goal, this program group has focused to establish (1) DNA-recognition module, (2) application of genome editing, and (3) delivery system, and to build (4) intellectual property strategy for genome editing. In this year, which is first year of this program, this R&D group has been conducted mainly the set-up of the facility and biological materials.

Subject 1: Establishment of DNA recognition module
(Tokyo Medical and Dental Univ., Kobe Univ., Tokyo Univ., AIST, RIKEN, EditForce Inc.)
Content & Result: This subject is aimed to (1) piratical use of PPR protein in genome editing, and (2) establishment of novel DNA recognition module. In this year, following R&D have been conducted.
- Staring the establishment of high-throughput assay systems to evaluate dPPR motifs.
- Design and construction of artificial, modified nucleic acid for DNA recognition, and performing trial test for the evaluation.
- Integration of adenine residue into amino acid to construct novel protein-nucleic acid hybrid module.
- Systematic survey of protein- and RNA-based novel genome editing module using database and newly developed software, and set-up the biomaterials for the evaluation.
- Trial in vitro verification of available protein-RNA complex for its use in genome editing.

Subject 2: Application of genome editing
(Kyushu Univ., Tokyo Medical and Dental Univ., Kobe Univ., Hiroshima Univ., RIKEN)
Content & Result: This subject is aimed to establish arious genome modification techniques. In this year, following R&D have been conducted.
- Optimization of delivery of genome editing tools using electroporation.
- Trail application of RNA-binding PPR based translational activation tools in plant cultured cell.
- Staring the establishment of in vitro evolution system of recombinase domain.
- Optimization of the artificial enzyme for system for efficient knock-in, semi-rational mutagenesis, accelerated homogenization of multiple genome copies.
- Initial survey of novel DNA cleavage enzyme.
- Optimization of the length of homologous sequences for mitochondrial genome editing. Construction of 6 plasmid vectors for chloroplast and mitochondrial genome editing.

Subject 3: Delivery system
(AIST)
Content & Result: This subject is aimed to establish novel delivery system for genome editing modules. In this year, following R&D have been conducted.
- Construction of reporter system for quantitative evaluation of genome editing efficiency, and its trial use in mammalian cultured cell.

Subject 4: Intellectual property strategy
(IPSN, Inc.)
Content & Result: This subject is to build intellectual property strategy for genome editing for the practical industrial use. In this year, following activities have been conducted.
- Accomplishment of the first report for the prior art of research about genome editing related to this program.
- Conclusion of the Unified Agreement of Intellectual Property among 13 institutes/companies participating in this program.
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