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成果報告書詳細
管理番号20170000000566
タイトル*平成28年度中間年報 植物等の生物を用いた高機能品生産技術の開発 植物の生産性制御に係る共通基盤技術開発 遺伝子発現制御および栽培環境制御の融合による代謝化合物高生産基盤技術開発 その1
公開日2017/7/6
報告書年度2016 - 2016
委託先名国立大学法人千葉大学
プロジェクト番号P16009
部署名材料・ナノテクノロジー部
和文要約
英文要約In this study, we evaluated the effects of UV light addition, ozone gas exposure, and
low nutrient solution temperature on the accumulation of functional phytochemicals and
antioxidant capacity in the leaves of rapeseed (Brassica napus L.) and Nicotiana
benthamiana plants under controlled environments.
Brassica napus L. and Nicotiana benthamiana grown under white LED light in a
controlled-environment room were used as the plant materials and tested under a given
stress condition for 3ー5 d prior to harvest.
Fluorescent UV-A&B lamps were used as the UV light source. PPFD was set at 200
ーmolーm-2ーs-1 by white LEDs. Ozone gas was exposed to the plants in a growth chamber
using an ozone gas control system developed by the authors. Ozone concentration was
set at 200 and 400 ppb. Nutrient solution temperature was controlled at 5, 10, 15, 20 and
22 (control) °C under air temperature of 22°C.
The Oxygen radical absorbance capacity (ORAC) value of Brassica napus L. leaf
exposed to 0.6 Wーm-2 for 5 d increased by 30ー40% compared to the control. There was
no different among the treatments. The ORAC values of the plants exposed to 200 and
400 ppb of ozone gas conc. were higher than that of the control. There was no different
among the treatments. The ORAC values of the plants at 10°C was higher than that of
the control. There was no different among the treatments.
The results indicate that UV-B light of appropriate intensity, ozone gas exposure of
appropriate intensity and low solution temperature with normal air temperature
stimulated the biosynthesis of bioactive compounds in plants. The results of DNA
microarray analysis support the change of the biosynthesis of secondary metabolites.
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