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成果報告書詳細
管理番号20170000000726
タイトル*平成28年度中間年報 植物等の生物を用いた高機能品生産技術の開発 植物の生産性制御に係る共通基盤技術開発 遺伝子発現制御および栽培環境制御の融合による代謝化合物高生産基盤技術開発
公開日2017/7/25
報告書年度2016 - 2016
委託先名国立研究開発法人産業技術総合研究所
プロジェクト番号P16009
部署名材料・ナノテクノロジー部
和文要約
英文要約Title: Development of Production Techniques for Highly Functional Biomaterials Using Smart Cells of Plants and Other Organisms. Development of technology for the regulation of DNA methylation of plant endogenous genes using CMV vectors and chromatin remodeling.(FY2016-FY2018)FY2016 Annual Report

The purpose of this project is to develop the technologies for the regulation of plant endogenous genes using DNA methylation. Chromatin structures are known to be closely related to the induction and maintenance of DNA-methylation activities. We presumed that several chromatin remodeling factors (CRFs) would induce the change of chromatin conformations and the activity of DNA methylation of plant endogenous genes. Accordingly, the CRFs coding genes of Arabidopsis thaliana and Nicotiana benthamiana were isolated and then cloned. We produced transgenic N. benthamiana plants expressing the CRF genes using Agrobacterium-mediated transformation. In addition, MeDIP (Methylated DNA immunoprecipitation) is being used to verify the induction of VIGS against all of the plant endogenous genes in CMV infected plants and/or Dicer-like (DCL)2 and 4 double knockdown plants.
The coding sequence and the putative promoter sequence of this project’s model gene, the PDS gene, were obtained from N. benthamiana. We attempted to induce post transcriptional gene silencing (PTGS) and RdDM-mediated transcriptional gene silencing (TGS) against the PDS gene using virus vectors and RNA interference technology. The results showed that, although PTGS was efficiently induced, induction of TGS was not visibly recognized, suggesting that TGS induction of the endogenous gene is not easy.


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