成果報告書詳細
管理番号100013719
タイトル*平成20年度中間年報 微生物機能を活用した環境調和型製造基盤技術開発/微生物機能を活用した高度製造基盤技術開発/バイオリファイナリー技術の開発(H18-21)
公開日2009/4/24
報告書年度2008 - 2008
委託先名財団法人地球環境産業技術研究機構
プロジェクト番号P06014
部署名バイオテクノロジー・医療技術開発部
和文要約以下本編抜粋:1.研究開発の内容及び成果等バイオリファイナリーは、再生可能資源であるバイオマス由来の糖類を原料としてバイオプロセスにより、有価化学品や燃料を生産する産業技術コンセプトである。本研究開発では、日本における経済性優位なるバイオリファイナリー産業の早期実現を目指し、独自の革新的なバイオプロセス「増殖非依存型バイオプロセス」をコア技術として、ソフトバイオマス由来の糖を原料とした基幹化学物質製造プロセスの工業化技術の確立を行う。
英文要約Title : Development of fundamental technology for environmentally friendly production using microbial cell functions/ Development of fundamental technology for advanced production using microbial cell functions/ Development of biorefinery technology (FY2006-FY2009) FY2008 Annual Report 1. Development of saccharification technology from soft biomass: With regards to development of highly-functional cellulases, we examined application of cellulosomes. When we cultured Clostridium in the presence of lignin-containing used paper as the sole carbon source, the composition of consequent cellulosomes was optimized for degradation of the substrate. A mixture of cellulosomes and a commercially available cellulase exhibited synergistic positive effects on saccharification of the substrate. In a basic research to impart cellulosome productivity in an industrially useful microorganism, we improved the heterogeneous protein secretion system of Corynebacterium glutamicum. We obtained mutants with high secretion ability, whose application made the protein expression system much more efficient. We also determined the crystal structure of a cellulase with high heat-resistance. 2. Development of growth-arrested bioprocess: When C. glutamicum cells are cultured under oxygen deprived conditions, the growth-arrested cells maintain their main metabolic capabilities. We investigate various cell functions related to this unique property of C. glutamicum in order to develop a new bioprocess leading to establishment of a biorefinery with superior cost competitiveness. In this fiscal year, we described new findings related to functions and regulation mechanisms of various genes involved in sugar uptake, product excretion, carbohydrate metabolism, redox balance, cell division and so on. Based on these findings, genetic engineering of regulation of sugar metabolism genes turned out to be an effective strategy for enhancement of the productivity of the growth-arrested bioprocess. We also identified a Corynebacterium strain which can grow on pentose sugars as sole carbon sources, and established that its pentose metabolism genes provide a very useful tool for establishment of simultaneous utilization of mixed sugars derived from biomass. In order to establish fundamental technologies for highly efficient production of biochemicals from soft biomass, we proceeded with metabolic engineering for production of various model chemicals. In addition to D-lactate and L-alanine whose highly efficient production processes we reported previously, in this fiscal year, we developed a growth-arrested bioprocess for xylitol production as a model intracellular coenzyme regenerating system, and high productivity was achieved.
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