成果報告書詳細
管理番号100013720
タイトル*平成20年度中間年報 微生物群のデザイン化による高効率型環境バイオ処理技術開発 システム論的アプローチによる微生物コミュニティーデザイン
公開日2009/4/24
報告書年度2008 - 2008
委託先名学校法人早稲田大学
プロジェクト番号P07024
部署名バイオテクノロジー・医療技術開発部
和文要約以下本編抜粋:本研究開発の内容本研究開発では、排水処理システム内の微生物コミュニティーを一つのシステムとして捉え、実験およびシミュレーションモデル解析を併用したシステム論的アプローチにより微生物コミュニティーのデザイン化を行う。
英文要約Title: Development of High-efficiency Environmental Biotreatment Technology Using Artificially Designed Microbial Communities / Microbial Community Design Using Novel Systematic Methodology (FY2007-FY2009) FY2008 Annual Report In this project, we aim to establish novel methodological approach, in which constructed model simulation results are critically compared with experimental results to calibrate model parameters and/or to refine the models, for designing microbial community that optimizes performance of biological wastewater treatment. As a model case applicable of this approach, simultaneous nitrogen and phosphorus removal process using microbial granular sludge is considered. In this process, nitrogen and phosphorus are removed simultaneously in a single reactor because nitrifying bacteria and denitrifying polyphosphate-accumulating organisms can coexist within the granular sludge. Because of this complexity, constructing a mathematical model for this process by considering interactions between solute and particulate species is essential to predict microbial community structure and/or to optimize the treatment performance. In fiscal 2008, we achieved the following four items. (1) Kinetic and stoichiometric parameters of the microorganisms involved essentially in the simultaneous nitrogen and phosphorus removal process were estimated with batch respirometric assays. In addition to the parameters of ammonia oxidizing bacteria (AOB) estimated in fiscal 2007, those of nitrite oxidation bacteria (NOB), heterotrophic bacteria (Het), and polyphosphate-accumulating organisms (PAOs) were estimated. (2) A simultaneous nitrogen and phosphorus removal system based on a laboratory-scale sequencing-batch reactor (SBR) was constructed and operated. (3) Existing ratios of AOB, NOB, Het, and PAO in inoculum were estimated by fluorescence in situ hybridization (FISH) analysis. (4) Two types of mathematical models, macroscopic and microscopic models, for the simultaneous nitrogen and phosphorus removal process were developed. While the macroscopic model involves dynamics of the number of granules in a reactor, the microscopic model can describe microbial community structure in the granules. Methodology for connecting these two models was also established.
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