成果報告書詳細
管理番号100013737
タイトル*平成20年度中間年報 「生物機能活用型循環産業システム創造プログラム・省エネルギー技術開発プログラム/植物機能を活用した高度モノづくり基盤技術開発/植物の物質生産プロセス制御基盤技術開発(植物の統括的な遺伝子発現制御機能の解析)」(H18-H21)
公開日2009/4/24
報告書年度2008 - 2008
委託先名独立行政法人産業技術総合研究所
プロジェクト番号P02001
部署名バイオテクノロジー・医療技術開発部
和文要約以下本編抜粋:(3)転写因子遺伝子の機能解析渇水耐性の向上あるいはフェノール性化合物の蓄積の低下といった機能への関与が示唆されたBZFあるいはERFファミリーに属する転写因子について、各種条件での生長・分化、ストレス応答、代謝系などに関する形質変化や遺伝子発現変化などの詳細な解析を行うと共に各転写因子遺伝子のパラログの機能解析などを行った。
英文要約Title : Development of Fundamental Technologies for Controlling the Material Production Process of Plants. Functional Analysis of Transcription Factors for the Coordinate Regulation of Plant Metabolic Pathways. (FY2006 - FY2009) FY2008 Annual Report It becomes evident that transcription factors play an important role for the control of growth and development in plants. Therefore, functional analysis of transcription factors is an important subject for the manipulation of plant traits and functional genomics in Arabidopsis has been undertaken to investigate transcription factors involved in the regulation of metabolic pathways and biomass production in plant. We generated transgenic Arabidopsis plants overexpressing transcription factor genes and analyzed phenotypic changes and expression of target genes in the transgenic plants. Several transcription factors that may be useful to improve production and utilization of plant biomass have been identified. Overexpression of BZF1 confers enlargement of vegetative growth, increase in viability under severe drought condition and improvement of irrigation water use efficiency in Arabidopsis. Overexpression of an ERF gene may confer extension of leaf longevity and the post-harvest storage life in plants. We also attempted to generate transgenic plants of Brassica napus in order to examine the functions of hopeful transgenic genes in crops. Furthermore, we established experimental systems to investigate changes in phenotypes related to drought tolerance, requirement of nitrate, or photosynthesis in transgenic Arabidopsis plants overexpressing transcription factors, and examined several transgenic plants. We found new candidates for improvement of productivity of plant biomass. We developed a novel gene silencing system, called Chimeric REpressor Gene-Silencing Technology (CRES-T), in which a chimeric repressor produced by fusion of a transcription factor to the plant-specific ERF associated Amphiphilic Repression (EAR) motif suppresses the target genes dominantly over the activity of endogenous and functionally redundant transcription factors. Using this CRES-T system we searched Arabidopsis transcription factors that related to metabolite production by analyzing gene expression profiling and investigated their function by converting into chimeric repressors. Succeeding to last year, we analyzed transcription factors that would regulate phenylpropanoid, lipid, and shikimate biosynthetic pathway. We analyzed whole metabolome of plants that express the chimeric repressor for transcription factor putatively related to these metabolic pathways. We analyzed four, seven and five transgenic lines provably related to phenylpropanoid, lipid, and shikimate pathway, respectively. One line exhibited resistance to 5-methyltryptophan. In addition, we are analyzing transcription factors (chimeric repressors) that could increase hyaluronic acid of tobacco culture cells that expressed hyaluronic acid biosynthetic gene.
ダウンロード成果報告書データベース(ユーザ登録必須)から、ダウンロードしてください。

▲トップに戻る