成果報告書詳細
管理番号100013740
タイトル*平成20年度中間年報 「微生物機能を活用した環境調和型製造基盤技術開発/微生物群のデザイン化による高効率型環境バイオ処理技術開発/厨房廃水処理用油脂分解バイオフィルムの高機能化・安定化のための微生物製剤導入技術の研究開発事業」(H19-H21)
公開日2009/4/24
報告書年度2008 - 2008
委託先名国立大学法人名古屋工業大学
プロジェクト番号P07024
部署名バイオテクノロジー・医療技術開発部
和文要約以下本編抜粋:(1)環境からの油脂分解微生物の分離、および微生物製剤の品質管理技術の確立グリーストラップ内の環境は、油脂の加水分解のためpHが6.0以下の弱酸性である。そこで、市販のグリーストラップ用微生物製剤を培養したところ、pH7.0では増殖できたがpH6.0で増殖可能なものは存在しなかった。
英文要約Title : Research Project to Develop Technologies for Introducing Microbial Materials for Functionalization and Stabilization of Biofilms Degrading Oils and Fats in Waste Water from the Kitchen (FY2007-FY2009) FY2008 Annual Report We have isolated from soil Burkholderia arbolis SL1B1, which secrets lipase and utilizes triacylglycerol as the sole carbon source. This strain metabolizes free fatty acids but hardly does glycerol produced from triacylglycerol by lipase. We have also isolated the Candida cylindracea SL1B2, as a symbiotic strain with the strain SL1B1. This strain SL1B2 does not secret lipase and therefore cannot hydrolyze triacylglycerol. Although the yeast cannot metabolize free fatty acid, but utilizes glycerol as the sole carbon source for growth. We carried out triacylglycerol degradation experiments by pure cultivation of the strain SL1B1 and mixed cultivation of the strains SL1B1 and SL1B2. Co-cultivation with C. cylindracea SL1B2 increased the rate of oil degradation by B. arbolis SL1B1 nearly 2-fold. Co-cultivation with B. arboris SL1B1 greatly enhanced the growth of C. cylindracea SL1B2 due to glycerol production by the bacterial strain. Thus, our results clearly demonstrated the symbiotic effectiveness of a glycerol-assimilating yeast SL1B2 and a lipase-secreting, fatty acid-assimilating bacterium SL1B1 on the enhancement of TAG degradation. Here, we should stress that this rate was demonstrated at pH 6.0. As mentioned later, this rate will jump at neutral pH. Not only the consumption rate of fatty acids but also the production rate of glycerol was faster in the mixed culture than that in the pure culture. This implies that the hydrolysis of TAG was enhanced although lipase activity was almost the same in the mixed and the pure cultures. This was clearly shown by the TLC analysis; more rapid production of fatty acids from TAG in the mixed culture was confirmed. The hydrolysis reaction of TAG into fatty acids and glycerol is a reversible reaction. By eliminating the reaction products the reaction is enhanced in the direction of hydrolysis. The same level of enzyme activity suggested that the hydrolysis of TAG was enhanced by the rapid consumption of glycerol by assimilating-microorganism. The other products, fatty acids, are rapidly removed from the culture broth by the lipase-secreting bacterium itself. It was shown that the optimum pH and temperature for lipase secreted by B. arbolis SL1B1 are pH 8.0 and 60 degrees, respectively. The thermal stability of lipase activity was also examined. Although the activity declined at 80 degrees or above, lipase did not lose its activity after incubation at 70 degrees for 2 hours. Thus, lipase secreted by B. arbolis SL1B1 showed high thermal stability.
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