成果報告書詳細
管理番号20090000000074
タイトル*平成20年度中間年報 新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発(先導技術開発) ポリアロマ系プラスチック原料の発酵生産システムの研究開発
公開日2009/7/30
報告書年度2008 - 2008
委託先名国立大学法人筑波大学
プロジェクト番号P07015
部署名新エネルギー技術開発部 バイオマスグループ
和文要約以下本編抜粋:1.研究開発の内容および成果等(1)研究概要 ポリアロマ系プラスチック原料の安定な生産系の開発はバイオリファイナリーの夢である。本研究開発では、提案者が独自に発見した3-フェニル乳酸生産菌および他の微生物を利用して、当該原料として有望な3-フェニル乳酸をグルコースを原料として10-20 g/Lの規模で大量生産するシステムを開発する。
英文要約Title: High Efficiency Bioenergy Conversion Project, Development of Preparatory Basic Bioenergy Technologies, Development of Fermentation System for Producing Aromatic Polymers (FY2008-FY2010) FY2008 Annual report: Developing new biomass-derived feedstocks is important in order to utilize limited petroleum resources and fossil fuels. It is expected that a fermentation system for biomass-derived aromatic compounds, which has yet to be put in practical use, will lead to the creation of new biomass-based products such as aromatic polymers. Aiming to establish a new fermentation system for producing phenyllactate by using uniquely-developed microorganisms, the production mechanism of phenyllactate will be analyzed. While screening and isolating new bacteria, breeding bacteria with a phenyllactate-producing enzyme will also be carried out to build an efficient production process. This year, we screened phenyllactate-producers among fungi and bacteria to find several species produced phenyllactate. They include lactic bacteria. We also found in the cell-free extracts of one of the phenyllactate-producing fungus distinct activity of phenylpyruvate reductase (PPR), which was involved in phenyllactate production. The genes for PPR were found in bacteria. We introduced the PPR gene to Escherichia coli under the control of a gene promoter. The strain produced pheyllactate in culture medium, indicating that phenyllactate-producing bacterium was developed. For maximum phenyllactate production, we investigated optimum conditions of the bacterial culture including culturing temperature, composition and pH of the medium, metal requirements, and aeration. We also used genetically-manipulated E. coli strains in which shikimate pathway was up-regulated as the host bacteria for production. To date, our system achieved as much as 5 g/L production of phenyllactate. More than 95% of the produced phenyllactate was 3-phenyllactate. We developed extraction and purification system of the product from culture media by which we obtained crystallized product with high purity. Production of 3-phenyllactate as much as 10 to 20 g/L is being developed.
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