成果報告書詳細
管理番号20090000000178
タイトル*平成20年度中間年報 SBIR技術革新事業/特定食物アレルゲンの迅速・簡易定量法の開発
公開日2009/7/31
報告書年度2008 - 2008
委託先名ITEA株式会社
プロジェクト番号P08015
部署名研究開発推進部 技術革新・基盤技術グループ
和文要約以下本編抜粋:(1) 市場調査 -1. 食物アレルゲン検査市場規模 食物アレルゲン検査市場は、2005 年の調査開始時以降、毎年1 割程度増加し、2013 年には十億
円を超える市場と予測されている。検査法はスクリーニング検査であるELISA 法、イムノクロマト法、確定検査であるウェスタンブロット法、PCR 法という検査で構成されており、イムノクロマト法は約30%を占める(2008 年)/出典:富士経済株式会社。
英文要約An investigative study on development of an assay system to detect fast and easily food allergens (FY2008-FY2009) FY2008 Annual Report
ITEA Inc. has carried out a feasible study on development of an assay system to detect 7 kinds of food allegens defined by Food Sanitation Law in Japan on The Small Business Innovative Research 2008. The aim of the study was to investigate whether development of an immunochromatography that could quickly, easily and quantitatively detect multiple food allergens on site was feasible or not in terms of technology and business. We conducted market research on food inspection. Based on a market report about food inspection (Fuji Keizai Co., Ltd.), total distribution price of immunochromatograpy in 2008 has been increasing 1.37 times from 2005. There is a trend that makers and sellers dealing with food products conduct food test by their self and demand easy to use and cheaper assay for the test. So, they have been replacing ELISA with immunochromatograpy for food antigen testing. We interviewed several companies making food products and found that they preferred immunochromatograpy to ELISA because of cheaper and ease to use, and they considered that semi-quantitative assay was found to be enough to confirm no contamination of food allergens. They were interested in an assay system that can survey multiple allergens at one test, because they want to decrease time and cost for testing. In scientific research, we could establish an immunochromatography for detecting multiple allergens such as chicken egg, cow milk, and crustacean with repeatability and reliability using specific antibodies against the allergens. It was found that the assay could detect these allergens from the concentration of 2.5 ppm. It took just 22 minutes that samples were applied and test results were judged. Any machinery didn’t be needed for measurement on the assay. With standard samples of known concentration, the assay could measure semi-quantitatively their concentration. We considered that theoretically, it is possible to measure more multiple allergens in one testing in this assay. These findings strongly suggest that our immunochromatography is a suitable assay system to detect quickly, easily and quantitatively multiple food allergens in foodstuffs on site and can meet the needs of company dealing with food products.
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