成果報告書詳細
管理番号20090000000184
タイトル*平成20年度中間年報 SBIR技術革新事業 特定食物アレルゲンの迅速・簡易定量装置実現可能性の調査
公開日2010/6/5
報告書年度2008 - 2008
委託先名マイクロ化学技研株式会社 国立大学法人東京大学 国立大学法人名古屋大学
プロジェクト番号P08015
部署名研究開発推進部 技術革新・基盤技術グループ
和文要約以下本編抜粋:1. 研究開発の内容及び成果等 食品における特定原材料7品目 卵、乳、小麦、そば、落花生、えび、かに)の含有検査において、従来法と比較して迅速、簡便、かつ安価に検出できる装置(以下本装置という)が、本受託者所有技術であるマイクロ化学技術(各種免疫分析チップ)および免疫分析装置によって技術的に実現可能か、また、事業として成立するか否かの検証を行うことを目的としている。
英文要約In this project, we confirmed the technical and economic feasibility of our technologies (various immunoassay chips and an automatic microchip-based enzyme-linked immunosorbent assay (microELISA system)) for the detection of 7 specific food ingredients (egg, milk, wheat, buckwheat, peanut, shrimp, and crab). First, we examined the detection of egg (ovalbumin) and milk (casein) proteins in food samples. These food proteins (provided by the National Institute of Health Sciences (NIHS)) were extracted by the standard method. The extracts were analyzed using our system with primary antibodies to these proteins immobilized on polystyrene microbeads (provided by NIHS), standard solution, commercially available horseradish peroxidase (HRP)-conjugated anti-ovalbumin, anti-casein polyclonal antibody as the second antibody, the substrate for HRP, and running buffer. We were able to detect each protein present at levels of < 1 μg in the 1-g sample in approximately 1 hour. Next, we investigated the technological trends in the detection of the abovementioned food ingredients and microchip technology. Immunoassay methods (microplate ELISA and immunochromatographic methods) are mainly used for food-allergen detection. This suggests that our system, which is based on the same principle, will be useful for the detection of food proteins. Commercially available antibodies to the above mentioned food proteins were used for our system. The present preparative method entailed problems such as complications in handling and the risk of contamination. In order to solve these problems, we developed disposable pretreatment devices. We developed the microchips on which antibodies could be immobilized directly, and designed microchips with which simultaneous determination of multiple proteins was possible. Moreover, we analyzed the market characteristics on the basis of primary and secondary information. The primary targets of our consumer base were large baking and seasoning industries. We found that there was a need to reduce the cost per inspection in order to facilitate higher test frequency and increase in the number of test items (up to a maximum of 25 items). The market size in 2012 is estimated to be approximately 1 billion yen, and a manyfold expansion is necessary to meet the above requirements. These results suggest that the development of an economical automatic inspection system for the detection of the specified ingredients would be very promising. We estimate that the sale value of this system, the reagents, and the supplies will be approximately 90 million yen in 2012 and 1 billion yen in 2016.
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