成果報告書詳細
管理番号20110000000589
タイトル*平成22年度中間年報 新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発(先導技術開発) イオン液体を利用したバイオマスからのバイオ燃料生産技術の開発
公開日2011/6/7
報告書年度2010 - 2010
委託先名国立大学法人神戸大学 株式会社豊田中央研究所 国立大学法人九州大学 トヨタ自動車株式会社
プロジェクト番号P07015
部署名新エネルギー部
和文要約和文要約等以下本編抜粋:1.研究開発の内容及び成果等 セルロース系バイオマスが新たな非可食性エネルギー原料として期待されている。しかし、セルロースは非常に結晶性が高い天然高分子で酵素分解速度は極めて遅いため、実用化の大きなボトルネックとなっている。本研究開発ではセルロースの結晶構造を緩和・脆化することができるイオン液体をバイオマスの前処理溶剤として用い、効率的な酵素分解およびエタノール発酵へと誘導する新プロセスの開発を目的とした。
英文要約Title:Development of Bio-fuel production technology by ionic liquid (FY2009-FY2010) FY2010 Annual Report
In this study, for deconstruction of biomass, the ionic liquid was used as a pre-treatment medium, and the effective degradation and assimilation procedure was investigated. This new and effective ethanol production process has been named as 'Ionic liquid-based Consolidated Bio-Processing (i-CBP)', and we make a consortium with 4 research institutes as follows:
(1) Development of ethanol fermentation process by one batch operation (arming yeast technology)
Firstly, four kinds celluloses, endoglucase (EG), cellobiohydrolase (CBH I & II), and beta-glucosidase (BGL) were simultaneously displayed on the surface of yeast. By using this cellulase displayed yeast strain, the ethanol fermentation from ionic liquid pre-treated biomass was performed. As a result, the highest ethanol production yield was attained at 30 oC. In the case of high temperature condition around at 50 oC, the biomass was only degradated to glucose by celluases on the yeast surface and the yeast could not convert to ethanol.
(2) Development of ethanol fermentation process by two batch operation
The optimal cellulose combination for ionic liquid pre-treated biomass was screened from 15 kinds of celluases, and the cocktail ratio was identified by cellulose saccharification rates. Subsequently, by using the mixture containing transgenic yeast strain which was secretary produced cellulase with optimal ratio, two batch processes was performed from ionic liquid pre-treated biomass. As a result, the ethanol production from biomass was confirmed without addition of crude cellulase solutions.
(3) Design and selection of ionic liquids for effective biomass pretreatment
By screening commercially available and synthesized ionic liquids, several potential ionic liquids were selected for lignocellulosic biomass in terms of cellulose crystalline structure and enzymatic oligosaccharide formation from the regenerated biomass. The ionic liquid pretreatment could decrease the cellulose crystalinity and enhance cellulase-catalyzed saccharification rates. The difference in functional group of ionic liquid affected the oligosaccharaide formation. Yields of regenerated biomass were also influenced by the pretreatment time and the kinds of ionic liquids and biomass employed.
(4) Development of ionic liquid recovery and recycle
In the case of two batch process, the recovery of ionic liquid through wash step from biomass was critical. As a wash medium, an ethanol, acetone and water was used for extraction of ionic liquid from biomass. By calculating of recovery yield with wash medium, the acetone and ethanol was attained 95 % over recovery of ionic liquid from pre-treated biomass. By increasing of repeating operation with wash medium, the coloring in ionic liquid was observed. However, there was no significant decreasing of enzymatic degradation efficiency.
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