成果報告書詳細
管理番号20110000000593
タイトル*平成22年度中間年報 新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発(先導技術開発) エネルギー植物の品種改良に係わる代謝情報と遺伝子発現情報に関する研究開発
公開日2011/6/29
報告書年度2010 - 2010
委託先名財団法人かずさディー・エヌ・エー研究所 独立行政法人理化学研究所 国立大学法人京都大学
プロジェクト番号P07015
部署名新エネルギー部
和文要約和文要約等以下本編抜粋:1.研究開発の内容及び成果等 (1)ギガシークエンサーによるエネルギー植物の遺伝子発現情報の解析 エリアンサス(Erianthus)の遺伝子発現を、ギガシークエンサー454を用いて計測した。葉と茎からそれぞれRNAを抽出し、cDNAライブラリーを作製し、それぞれ約450Mbの配列情報を取得した。配列のアセンブルを行い、56024個のUnigenes配列を得たので、この情報からKaPPA-Viewシステムで表示するための代謝地図を作成した。
英文要約New Energy Technology Development Project / High Efficiency Bioenergy
Conversion Project, Development of Preparatory Basic Bioenergy
Technologies
Research and Development of Genetic and Metabolic Information on Energy
Producing Plants for Breeding Programs
Project Summary of FY 2010
 Many plant species have attracted attention as potential feedstocks for biofuel.
However, the available genetic and metabolic information on most of these plants
is, in general, limited. Few databases have been developed that provide both
genetic and metabolic data on energy producing plants in an integrated manner.
We will analyze genes and metabolites of energy producing plants such as
Erianthus, switchgrass, miscanthus, poplar, jatropha and oil-producing algae in a
comprehensive manner and then develop databases of the information obtained.
 Analysis of Gene Expressions and Low-solubility substances
 Gene expression of Erianthus, switchgrass, jatropha and oil-producing algae
was examined by giga-sequencers. Soluble metabolites of these plants were
analyzed by mass-spectrometry. In addition to these gene expression of soluble
metabolite analyses, we have investigated solubilization of low-solubility biomass
such as lignin and hemicelluloses using ball-milling and a choice of adequate
solvent systems. As a result, we have succeeded in obtaining nicely resolved 1H-
13C correlation spectra of low-solubility cell-wall components from biomass
plants such as erianthus, switchgrass and jatropha.
 Analysis of Lignin of Energy Producing Plants
 Lignin is a major component of the secondary cell wall of vascular plants, and
is an obstacle in the conversion of plant cell wall polysaccharides into biofuels.
However, little is known about lignins of energy-producing plants. In the last
fiscal year, we established a microscale, high-throughput, and highly reproducible
nitrobenzene oxidation method and a method for characterizing p-
hydroxycinnamic acids, which also inhibit enzymatic saccharification of
secondary cell wall polysaccharides. In this fiscal year, we have successfully
applied these methods to the analysis of a number of typical energy-producing
plants, thereby lignins and related compounds of the plants have been
characterized.
 Construction of Database on Energy Producing Plant Metabolites
 To accumulate a large amount of data obtained from energy producing plants in
this study, we constructed a database KaPPA-Loader. To view the datasets on
metabolic pathway maps, we prepared KEGG-formatted metabolic maps for the
energy producing plants we analyzed, and then integrated them into the KaPPA-
View system.
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