成果報告書詳細
管理番号20110000000928
タイトル*平成22年度中間年報 バイオマスエネルギー技術研究開発 戦略的次世代バイオマスエネルギー利用技術開発事業(次世代技術開発) 共生を利用した微細藻類からのバイオ燃料製造プロセスの研究開発
公開日2011/6/29
報告書年度2010 - 2010
委託先名国立大学法人筑波大学 JFEエンジニアリング株式会社
プロジェクト番号P10010
部署名新エネルギー部
和文要約和文要約等以下本編抜粋:1.研究開発の内容及び成果等 (1)微細藻類の培養技術に関する基礎検討 ●共生微生物の探索 炭化水素を産生する緑藻類Botryococcus braunii(以下ボトリオコッカス)の増殖を促進させる共生微生物の探索を実施した。ミャンマーのバゴー市のため池で大発し、ブルームを形成していたボトリオコッカスを約30株分離培養した中から、バクテリアを緊密な共生関係をもっていると思われる培養株TanaBot-Ba10を得ることができた。光学顕微鏡で藻体のコロニーを押しつぶすと、多量のオイルがコロニーの周りに分泌される(図1)。
英文要約Development of biomass fuel manufacturing process from microalgae using symbiosis" (FY2010~FY2011)FY2010 Annual Report
1.Culturing Techniques for microalgae:(1)Screening of symbiotic bacteria:Despite the potential utility of mutualistic relationships between algae and bacteria to promote the growth of algal culture, symbiotic bacteria have rarely been explored for Botrycoccus. In this study, we have identified a possible ectosymbiotic bacterium form a strain of Botrycoccus braunii. This bacterium cannot grow in the standard medium for heterotrophic bacteria. Culture-independent DNA sequence analysis using the ribosomal RNA gene indicated that this bacterium is affiliated into a clade consisting of uncultured bacterial sequences within α-proteobacteria. In silico survey of the DNA databank revealed that most of members belonging to this phylogroup are isolates from eutrophic water environments. A specific association of some members with bloom-forming algae was also suggested.:(2)Effects of soybean curd wastewater on the growth of Euglena gracilis.:A laboratory study was conducted to evaluate the possibility of using wastewater from a soybean curd manufacturing plant as a growth promoter of Euglena gracilis. Varying amounts of the soybean curd wastewater (SCW) were added to AF-6 medium to set the final concentration of the wastewater to 0 (control), 10, 20, 50, and 100% (v/v). The growth observed in the cultures with 100% SCW were approximately 10 times higher than that observed in the control. On the other hand, the growth of Botryococcus braunii was inhibited by the concentration of SCW with more than 5% . It seems that the primary treatment and the secondary treatment of SCW can be efficiently utilized by using E. gracilis and B braunii, respectively.:2.Studies on algae oil manufacturing system:(1)Cultivation plants:A) Open pond Advantages:・Low cultivation cost Disadvantages:・Weak protection from contamination・Poor control B)Photo-bioreactor(PBR) Advantages:・high productivity, good control(easy prohibit of contaminations)・Large ratio of surface/volume・High concentration of micro algae (3 - 5 times compared with cultivation by open pond ) Disadvantages:・Very expensive equipment cost・High operation cost.:(2)Micro algae strains for oil production:As microalgae strains for biofuel production, a)Botryococcus、b)Chlorella、c)Euglena、d)Nannochloropsis、e)Scenedesmus etc. have been reported and they can grow both in autotrophic and heterotrophic condition. Heterotrophic cultivation is that algae increase by using organic substances in wastewater as energy source. Then it is considered that it is possible to get large energy by using wastewater.:(3)Biomass fuel manufacturing process and construction of cultivation reactor:It was decided that PBR was adopted in this research project to aiming at high throughput (L/m2/y) and that the capacity of experimental equipment was 100L ,which will be able to make the growth rate higher by controlling the water temperature (25 - 35℃)and pH (neutral to slight alkali).
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