本文へジャンプ

成果報告書詳細
管理番号20180000000150
タイトル*平成29年度中間年報 植物等の生物を用いた高機能品生産技術の開発 植物の生産性制御に係る共通基盤技術開発 ゲノム編集の国産技術基盤プラットフォームの確立
公開日2018/6/26
報告書年度2017 - 2017
委託先名エディットフォース株式会社 国立大学法人東京医科歯科大学 国立大学法人神戸大学 国立研究開発法人産業技術総合研究所 国立大学法人広島大学 国立研究開発法人理化学研究所 国立大学法人九州大学 国立大学法人東京大学 知的財産戦略ネットワーク株式会社
プロジェクト番号P16009
部署名材料・ナノテクノロジー部
和文要約
英文要約Summary for report 2017
Research & Developmental Program
Development of bio-production technologies of valuable substances using plants
- Development of basic and fundamental technologies for bio-production using plant
- Establishment of Japanese technological platform of genome editing
(Kyushu Univ., Tokyo Medical and Dental Univ., Kobe Univ., Hiroshima Univ., Tokyo Univ., AIST, RIKEN, EditForce Inc., IPSN, Inc.)

Summary: This program has been launched to establish fundamental, independent technological platform of “genome editing” and its use in bio-production area, to improve the international industrial competitiveness for Japan. This program has also aimed the practical use of newly established genome editing technologies for production of valuable substances using plants. To achieve this goal, this program group has focused to establish (1) DNA-recognition module, (2) application of genome editing, and (3) delivery system, and to build (4) intellectual property strategy for genome editing. In this year, which is first year of this program, this R&D group has been conducted mainly the set-up of the facility and biological materials.

Subject 1: Establishment of DNA recognition module
(Tokyo Medical and Dental Univ., Kobe Univ., Tokyo Univ., AIST, RIKEN, EditForce Inc.)
Content & Result: This subject is aimed to (1) piratical use of PPR protein in genome editing, and (2) establishment of novel DNA recognition module. In this year, following R&D have been conducted.
- Proof-of-Concept of genome editing using dPPR motifs was obtained.
- Evaluation of artificial, modified nucleic acid for DNA recognition and catalysis.
- Integration of cytosine residue into amino acid to construct novel protein-nucleic acid hybrid module.
- Refinement of the software for systematic survey of protein- and RNA-based novel genome editing module using database, and obtaining the candidates.
- In vitro verification of available protein-RNA complex for its use in genome editing.

Subject 2: Application of genome editing
(Kyushu Univ., Tokyo Medical and Dental Univ., Kobe Univ., Hiroshima Univ., RIKEN)
Content & Result: This subject is aimed to establish various genome modification techniques. In this year, following R&D have been conducted.
- Optimization of delivery of genome editing tools using electroporation.
- Evaluation of RNA-binding PPR based translational activation tool and splicing tool in plant cultured cell.
- Staring the artificial evolution system of recombinase domain using yeast.
- Optimization of the artificial enzyme for system for efficient knock-in, semi-rational mutagenesis, accelerated homogenization of multiple genome copies.
- Detailed analyses of novel DNA cleavage enzymes.
- Obtaining chloroplast and mitochondrial genome editing tobacco plants.

Subject 3: Delivery system
(AIST)
Content & Result: This subject is aimed to establish novel delivery system for genome editing modules. In this year, following R&D have been conducted.
- Obtaining proof-of-concept for physicochemical delivery system in plant cultured cell.

Subject 4: intellectual property strategy
(IPSN, Inc.)
Content & Result: This subject is to build intellectual property strategy for genome editing for the practical industrial use. In this year, following activities have been conducted.
- Composition of 2nd report for the about genome editing related to this program.
- Starting on IP portfolio composition
- IP management committee (2 times), and approved new 2 IP applications
ダウンロード成果報告書データベース(ユーザ登録必須)から、ダウンロードしてください。

▲トップに戻る