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成果報告書詳細
管理番号20190000000330
タイトル*平成30年度中間年報 次世代人工知能・ロボット中核技術開発 革新的ロボット要素技術分野 人検知ロボットのための嗅覚受容体を用いた匂いセンサの開発
公開日2019/6/27
報告書年度2018 - 2018
委託先名国立大学法人東京大学 住友化学株式会社 地方独立行政法人神奈川県立産業技術総合研究所
プロジェクト番号P15009
部署名ロボット・AI部
和文要約
英文要約Title: Development of Odor Sensor Using Olfactory Receptors for Human Searching (FY2015-FY2019) FY2018 Annual Report

Sumitomo Chemical Company, Kanagawa Institute of Industrial Science and Technology (KISTEC), and The University of Tokyo set higher agendas for the final goal in this project aiming to develop the odorant biosensor. Sumitomo Chemical Company worked on producing a durable and sensitive olfactory receptor (OR) with OR co-receptor (Orco). KISTEC examined a cell-free protein synthesis system and solution exchange system. The University of Tokyo examined the configurations for suppressing the noise and the array of the chips. These three institutes obtained sufficient progresses in this project.

The group of Sumitomo Chemical Company has attempted to develop a durable sensor protein for the long-term use of the odor sensing device. Hundreds of genetically modified Orcos were constructed by a single amino acid replacement. The mutated Orco proteins were purified and subjected to a stability assessment, and more stable proteins were obtained. Furthermore, to improve the signal acquisition of odorant sensor, the previous method was modified to produce nano-sized uniform proteoliposomes, and reproducible electrical signals were obtained with the proteoliposomes.

KISTEC group introduced a cell-free protein synthesis system to obtain OR-Orco with more simple procedures. To verify the sensor containing OR-Orco produced by the cell-free system, octenol gas detection was conducted. The odorant sensor successfully detected octenol gas at 0.01ppm within 30 min. In addition, the group addressed the potential of a liquid flowing system driven by a superabsorbent polymer for removal of accumulated odorant in the sensor chip, realizing detection of liquid changes in analyte concentration. The system is pumpless and therefore requiring no electricity. Experimentally, liquid exchange was demonstrated that reduction of cyclodextrin (analyte) concentration was monitored in real time by using the superabsorbent polymer. The system will facilitate the portability and usability of the odorant sensors.

The group of The University of Tokyo developed a portable readout device that suppresses the influence of environmental current noise. The current noise is a critical issue for the odorant sensor because of the minute signal from the receptor. We found enormous noise on our previous device without the Faraday shield, while the developed one with the socket-pin connection was relatively unaffected by the noise even without the shield, suitable for gas sensing. In addition, we integrated multiple readout circuits into a single portable device to realize odorant detection with multiple channels in future.
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